Drosophila Melanogaster, an Emerging Animal Model for the Study of Human Cardiac Diseases

Background: The need to work with model organisms in medical research has revealed the usefulness of the fruit fly Drosophila melanogaster, considering its advantages to perform classic genetic studies and modern techniques of genome edition. Several human genes are similar to those of the fruit fly...

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Autores principales: Santalla, Manuela, Portiansky, Enrique Leo, Ferrero, Paola Viviana
Formato: Articulo
Lenguaje:Inglés
Publicado: 2016
Materias:
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/80585
http://ppct.caicyt.gov.ar/index.php/rac/article/view/8711
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id I19-R120-10915-80585
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Médicas
Drosophila melanogaster
Transgenesis
Calcium
Caffeine
Calcio
Cafeína
spellingShingle Ciencias Médicas
Drosophila melanogaster
Transgenesis
Calcium
Caffeine
Calcio
Cafeína
Santalla, Manuela
Portiansky, Enrique Leo
Ferrero, Paola Viviana
Drosophila Melanogaster, an Emerging Animal Model for the Study of Human Cardiac Diseases
topic_facet Ciencias Médicas
Drosophila melanogaster
Transgenesis
Calcium
Caffeine
Calcio
Cafeína
description Background: The need to work with model organisms in medical research has revealed the usefulness of the fruit fly Drosophila melanogaster, considering its advantages to perform classic genetic studies and modern techniques of genome edition. Several human genes are similar to those of the fruit fly. We have developed for the first time in the country a cardiovascular line of research to study the genetics of aging, addictions and chronic consumption of substances in humans like caffeine. Objective: The aim of this study was to provide experimental evidence that validates Drosophila melanogaster as a model to study human cardiomyopathies related to the pharmacological action of caffeine on the heart. Methods: Cardiac function and the effect of caffeine were studied in semi-intact preparations of Drosophila melanogaster. Heart rate and the intracellular calcium transient were recorded and analyzed in 3, 7 and 40-day-old adult flies harboring one genetically encoded reporter system. Hearts of adult flies were dissected to show the myofibrillar structural organization and specific proteins such as SERCA. Results: Aging and caffeine alter contraction rate and intracellular calcium handling in the adult heart of Drosophila melanogaster in a similar way as mammals Conclusion: The study supports the use of this model of fast and easy reproductive cycle to identify the genes involved in the mechanisms through which aging, caffeine (and other substances) and environmental factors affect the heart.
format Articulo
Articulo
author Santalla, Manuela
Portiansky, Enrique Leo
Ferrero, Paola Viviana
author_facet Santalla, Manuela
Portiansky, Enrique Leo
Ferrero, Paola Viviana
author_sort Santalla, Manuela
title Drosophila Melanogaster, an Emerging Animal Model for the Study of Human Cardiac Diseases
title_short Drosophila Melanogaster, an Emerging Animal Model for the Study of Human Cardiac Diseases
title_full Drosophila Melanogaster, an Emerging Animal Model for the Study of Human Cardiac Diseases
title_fullStr Drosophila Melanogaster, an Emerging Animal Model for the Study of Human Cardiac Diseases
title_full_unstemmed Drosophila Melanogaster, an Emerging Animal Model for the Study of Human Cardiac Diseases
title_sort drosophila melanogaster, an emerging animal model for the study of human cardiac diseases
publishDate 2016
url http://sedici.unlp.edu.ar/handle/10915/80585
http://ppct.caicyt.gov.ar/index.php/rac/article/view/8711
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