A quantitative method using one marker for simultaneous assay of steroidal saponins in rhizoma paridis
Current quality control patterns are limited to industrial application, for most the natural chemical reference substances are expensive and unavailable. Here in, quantitative analysis of multi-components with single marker (QAMS) method, is established and validated to simultaneously determine five...
Guardado en:
| Autores principales: | , , , , , |
|---|---|
| Formato: | Articulo |
| Lenguaje: | Inglés |
| Publicado: |
2010
|
| Materias: | |
| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/8045 http://www.latamjpharm.org/resumenes/29/7/LAJOP_29_7_1_18.pdf |
| Aporte de: |
| Sumario: | Current quality control patterns are limited to industrial application, for most the natural chemical reference substances are expensive and unavailable. Here in, quantitative analysis of multi-components with single marker (QAMS) method, is established and validated to simultaneously determine five steroidal saponins (Paris-VII, Paris-H, Paris-II, Dioscin , Paris-I) in Rhizoma Paridis. Using Paris-I as the contrast, the relative correction factors (RCF) of the other four steroidal saponins are determined by HPLC-UV. With in the linear ranges, the values of RCF of Paris-I to Paris-VII, Paris-H, Paris-II and Dioscin are 0.877, 1.087, 0.975 and 1.127, respectively. The RCF has a good reproducibility in various instruments, chromatographic columns (RSD = 0.88 % ~ 4.52 %). According to their RCF, five steroidal saponins are simultaneously determined in Rhizoma Paridis by one marker. |
|---|