Inhibition of tumor cell proliferation and induction of apoptosis in 95-D lung cancer cells by drimartol A from hairy root cultures of Artemisia annua

Drimartol A (DA), a sesquiterpene courmarin ether, was isolated from the cultured hairy roots of A. annua for the first time, and no biological activity of DA has ever been reported. In this work, DA was shown to possess interesting cytotoxic activities against the human tumor cell lines of HO8910 (...

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Autores principales: Zhai, Dan-Dan, Supaibulwatana, Kanyaratt, Zhong, Jian-Jiang
Formato: Articulo
Lenguaje:Inglés
Publicado: 2010
Materias:
Farmacia
sesquiterpene courmarin ether; cytotoxicity; apoptosis; Artemisia annua; plant hairy root culture
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/8043
http://www.latamjpharm.org/resumenes/29/7/LAJOP_29_7_1_16.pdf
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-8043
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Farmacia
sesquiterpene courmarin ether; cytotoxicity; apoptosis; Artemisia annua; plant hairy root culture
spellingShingle Farmacia
sesquiterpene courmarin ether; cytotoxicity; apoptosis; Artemisia annua; plant hairy root culture
Zhai, Dan-Dan
Supaibulwatana, Kanyaratt
Zhong, Jian-Jiang
Inhibition of tumor cell proliferation and induction of apoptosis in 95-D lung cancer cells by drimartol A from hairy root cultures of Artemisia annua
topic_facet Farmacia
sesquiterpene courmarin ether; cytotoxicity; apoptosis; Artemisia annua; plant hairy root culture
description Drimartol A (DA), a sesquiterpene courmarin ether, was isolated from the cultured hairy roots of A. annua for the first time, and no biological activity of DA has ever been reported. In this work, DA was shown to possess interesting cytotoxic activities against the human tumor cell lines of HO8910 (ovary), 95-D (lung), QGY (liver) and HeLa (cervix) by MTT assay, whose IC50 values were ranged within 17.94-22.3 μM for 24h. Given that treatment of lung cancer is a priority of our interest, induction of apoptosis by DA in the human lung tumor cell line 95-D was focused. The 95-D cell growth was inhibited in a time dependent manner and its cell cycle was arrested in the G2 phase by DA. The apoptotic rate of the cells increased in a dose-dependent manner. DA also increased the activity of caspase-9 and -3 and caused a decrease in the mitochondrial membrane potential. These results revealed that DA could efficiently induce 95-D cell apoptosis through mitochondrial dependent pathway, and it may be a potential chemotherapeutic agent.
format Articulo
Articulo
author Zhai, Dan-Dan
Supaibulwatana, Kanyaratt
Zhong, Jian-Jiang
author_facet Zhai, Dan-Dan
Supaibulwatana, Kanyaratt
Zhong, Jian-Jiang
author_sort Zhai, Dan-Dan
title Inhibition of tumor cell proliferation and induction of apoptosis in 95-D lung cancer cells by drimartol A from hairy root cultures of Artemisia annua
title_short Inhibition of tumor cell proliferation and induction of apoptosis in 95-D lung cancer cells by drimartol A from hairy root cultures of Artemisia annua
title_full Inhibition of tumor cell proliferation and induction of apoptosis in 95-D lung cancer cells by drimartol A from hairy root cultures of Artemisia annua
title_fullStr Inhibition of tumor cell proliferation and induction of apoptosis in 95-D lung cancer cells by drimartol A from hairy root cultures of Artemisia annua
title_full_unstemmed Inhibition of tumor cell proliferation and induction of apoptosis in 95-D lung cancer cells by drimartol A from hairy root cultures of Artemisia annua
title_sort inhibition of tumor cell proliferation and induction of apoptosis in 95-d lung cancer cells by drimartol a from hairy root cultures of artemisia annua
publishDate 2010
url http://sedici.unlp.edu.ar/handle/10915/8043
http://www.latamjpharm.org/resumenes/29/7/LAJOP_29_7_1_16.pdf
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AT supaibulwatanakanyaratt inhibitionoftumorcellproliferationandinductionofapoptosisin95dlungcancercellsbydrimartolafromhairyrootculturesofartemisiaannua
AT zhongjianjiang inhibitionoftumorcellproliferationandinductionofapoptosisin95dlungcancercellsbydrimartolafromhairyrootculturesofartemisiaannua
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