In vitro evaluation of genotoxicity of watercress extract through effect biomarkers considering human intake

Rorippa nasturtium-aquaticum (watercress) is recognized as a nutritious plant, rich in vitamins and minerals and it is used in the treatment of many illnesses. The aim of the present work was to develop a genotoxic analysis of this diet component considering human intake by means of effect biomark...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Casanova, Natalia A., López Nigro, Marcela M., Curieses, Silvana P., Carballo, Marta A.
Formato: Articulo
Lenguaje:Inglés
Publicado: 2010
Materias:
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/8037
http://www.latamjpharm.org/resumenes/29/7/LAJOP_29_7_1_10.pdf
Aporte de:
id I19-R120-10915-8037
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Farmacia
brassicaceae; genetic toxicology; rorippa nasturtium-aquaticum
spellingShingle Farmacia
brassicaceae; genetic toxicology; rorippa nasturtium-aquaticum
Casanova, Natalia A.
López Nigro, Marcela M.
Curieses, Silvana P.
Carballo, Marta A.
In vitro evaluation of genotoxicity of watercress extract through effect biomarkers considering human intake
topic_facet Farmacia
brassicaceae; genetic toxicology; rorippa nasturtium-aquaticum
description Rorippa nasturtium-aquaticum (watercress) is recognized as a nutritious plant, rich in vitamins and minerals and it is used in the treatment of many illnesses. The aim of the present work was to develop a genotoxic analysis of this diet component considering human intake by means of effect biomarkers such as mitotic index, cellular proliferation kinetic, sister chromatid exchanges, micronucleus frequency and single cell gel electrophoresis (comet assay), allowing to establish risk-benefit in the consumption of this vegetable. The cress plant was chopped; the obtained product was centrifuged, filtered (0.22 µm) and conserved to -20 ºC. Two concentrations of the aqueous extract (13.2-26.4 mg/ml of culture) were assayed in human peripheral blood culture of healthy volunteers. No statistically significant differences were detected in our experimental conditions for any of the evaluated biomarkers (p > 0.05). These findings indicate that the watercress extract would not induce cytotoxicity, chromosomal instability, clastogenicity or DNA double and single strand breaks or alkali-labiles sites. Hence, monitoring the toxicogenetic activity of different aqueous extracts would provide a better understanding of their potential ability to improve healthiness or prevent DNA damage.
format Articulo
Articulo
author Casanova, Natalia A.
López Nigro, Marcela M.
Curieses, Silvana P.
Carballo, Marta A.
author_facet Casanova, Natalia A.
López Nigro, Marcela M.
Curieses, Silvana P.
Carballo, Marta A.
author_sort Casanova, Natalia A.
title In vitro evaluation of genotoxicity of watercress extract through effect biomarkers considering human intake
title_short In vitro evaluation of genotoxicity of watercress extract through effect biomarkers considering human intake
title_full In vitro evaluation of genotoxicity of watercress extract through effect biomarkers considering human intake
title_fullStr In vitro evaluation of genotoxicity of watercress extract through effect biomarkers considering human intake
title_full_unstemmed In vitro evaluation of genotoxicity of watercress extract through effect biomarkers considering human intake
title_sort in vitro evaluation of genotoxicity of watercress extract through effect biomarkers considering human intake
publishDate 2010
url http://sedici.unlp.edu.ar/handle/10915/8037
http://www.latamjpharm.org/resumenes/29/7/LAJOP_29_7_1_10.pdf
work_keys_str_mv AT casanovanataliaa invitroevaluationofgenotoxicityofwatercressextractthrougheffectbiomarkersconsideringhumanintake
AT lopeznigromarcelam invitroevaluationofgenotoxicityofwatercressextractthrougheffectbiomarkersconsideringhumanintake
AT curiesessilvanap invitroevaluationofgenotoxicityofwatercressextractthrougheffectbiomarkersconsideringhumanintake
AT carballomartaa invitroevaluationofgenotoxicityofwatercressextractthrougheffectbiomarkersconsideringhumanintake
bdutipo_str Repositorios
_version_ 1764820486897795072