Microvesicle release and micellar attack as the alternative mechanisms involved in the red-bloodcell-membrane solubilization induced by argininebased surfactants

Two novel arginine-based surfactants, Bz-Arg-NHC10 and Bz-Arg-NHC12, were characterized with respect to surface properties and their interaction with human red-blood-cell (HRBC) membranes. The values for critical micellar concentration (CMC), the maximum surfactant adsorption at the air–liquid inter...

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Detalles Bibliográficos
Autores principales: Fait, María Elisa, Hermet, Melisa, Comelles, Francesc, Clapés, Pere, Álvarez, Hugo Ariel, Prieto, Eduardo Daniel, Herlax, Vanesa Silvana, Morcelle del Valle, Susana Raquel, Bakás, Laura Susana
Formato: Articulo
Lenguaje:Inglés
Publicado: 2017
Materias:
Biología
human red-blood-cell
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/80273
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-80273
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Biología
human red-blood-cell
spellingShingle Biología
human red-blood-cell
Fait, María Elisa
Hermet, Melisa
Comelles, Francesc
Clapés, Pere
Álvarez, Hugo Ariel
Prieto, Eduardo Daniel
Herlax, Vanesa Silvana
Morcelle del Valle, Susana Raquel
Bakás, Laura Susana
Microvesicle release and micellar attack as the alternative mechanisms involved in the red-bloodcell-membrane solubilization induced by argininebased surfactants
topic_facet Biología
human red-blood-cell
description Two novel arginine-based surfactants, Bz-Arg-NHC10 and Bz-Arg-NHC12, were characterized with respect to surface properties and their interaction with human red-blood-cell (HRBC) membranes. The values for critical micellar concentration (CMC), the maximum surfactant adsorption at the air–liquid interface, and the area per molecule indicated better surface properties for Bz-Arg-NHC12. The observation of cylindrical worm-like aggregates of Bz-Arg-NHCn via atomic-force microscopy supported the predictions based on the value of the surfactant-packing parameter (SPP). Erythrocyte-membrane solubilization was effected by surfactant aggregates since cell lysis became evident at only surfactant concentrations above the CMC. Changes in HRBC shape observed at different surfactant concentrations led to the conclusion that a slow mechanism based on the insertion of surfactant monomers into the HRBC membrane, followed by a shedding of microvesicles was responsible for the hemolysis produced by both surfactants at the lower concentrations tested. In contrast, the extraction of membrane lipids upon collisions between HRBCs and surfactant aggregates competes with and prevents microvesicle release at the higher concentrations assayed.
format Articulo
Articulo
author Fait, María Elisa
Hermet, Melisa
Comelles, Francesc
Clapés, Pere
Álvarez, Hugo Ariel
Prieto, Eduardo Daniel
Herlax, Vanesa Silvana
Morcelle del Valle, Susana Raquel
Bakás, Laura Susana
author_facet Fait, María Elisa
Hermet, Melisa
Comelles, Francesc
Clapés, Pere
Álvarez, Hugo Ariel
Prieto, Eduardo Daniel
Herlax, Vanesa Silvana
Morcelle del Valle, Susana Raquel
Bakás, Laura Susana
author_sort Fait, María Elisa
title Microvesicle release and micellar attack as the alternative mechanisms involved in the red-bloodcell-membrane solubilization induced by argininebased surfactants
title_short Microvesicle release and micellar attack as the alternative mechanisms involved in the red-bloodcell-membrane solubilization induced by argininebased surfactants
title_full Microvesicle release and micellar attack as the alternative mechanisms involved in the red-bloodcell-membrane solubilization induced by argininebased surfactants
title_fullStr Microvesicle release and micellar attack as the alternative mechanisms involved in the red-bloodcell-membrane solubilization induced by argininebased surfactants
title_full_unstemmed Microvesicle release and micellar attack as the alternative mechanisms involved in the red-bloodcell-membrane solubilization induced by argininebased surfactants
title_sort microvesicle release and micellar attack as the alternative mechanisms involved in the red-bloodcell-membrane solubilization induced by argininebased surfactants
publishDate 2017
url http://sedici.unlp.edu.ar/handle/10915/80273
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