A simple LC-ESI-MS method for the determination of norvancomycin in rat plasma and application to pharmacokinetic study

A simple and sensitive LC-ESI-MS method for determination of norvancomycin in plasma was developed and validated over the concentration range of 20-2,000 ng/mL. After addition of vancomycin as internal standard (IS), protein precipitation with 5 % trichloroacetic acid was employed for the sample...

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Detalles Bibliográficos
Autores principales: Yang, Xuezhi, Hu, Lufeng, Lin, Guanyang, Xu, Ren-ai, Zhang, Yu, Chen, Linzi, Zhu, Lihe, Zhao, Feng, Wang, Xianqin
Formato: Articulo Comunicacion
Lenguaje:Inglés
Publicado: 2010
Materias:
Farmacia
LC-ESI-MS; norvancomycin; rat plasma; pharmacokinetics
Antibióticos
Farmacología
Farmacocinética
Medicamentos
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/8024
http://www.latamjpharm.org/resumenes/29/6/LAJOP_29_6_2_9.pdf
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-8024
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Farmacia
LC-ESI-MS; norvancomycin; rat plasma; pharmacokinetics
Antibióticos
Farmacología
Farmacocinética
Medicamentos
spellingShingle Farmacia
LC-ESI-MS; norvancomycin; rat plasma; pharmacokinetics
Antibióticos
Farmacología
Farmacocinética
Medicamentos
Yang, Xuezhi
Hu, Lufeng
Lin, Guanyang
Xu, Ren-ai
Zhang, Yu
Chen, Linzi
Zhu, Lihe
Zhao, Feng
Wang, Xianqin
A simple LC-ESI-MS method for the determination of norvancomycin in rat plasma and application to pharmacokinetic study
topic_facet Farmacia
LC-ESI-MS; norvancomycin; rat plasma; pharmacokinetics
Antibióticos
Farmacología
Farmacocinética
Medicamentos
description A simple and sensitive LC-ESI-MS method for determination of norvancomycin in plasma was developed and validated over the concentration range of 20-2,000 ng/mL. After addition of vancomycin as internal standard (IS), protein precipitation with 5 % trichloroacetic acid was employed for the sample preparation. Chromatographic separation was performed on a Zorbax SB-C18 (100 mm×2.1 mm, 3.5 μm) column with 10:90 (v/v) acetonitrile-0.1 % formic acid as mobile phase. The MS data acquisition was accomplished by selective ions monitoring (SIM) mode with positive electrospray ionization (ESI) interface. The limit of quantification (LOQ) was 20 ng/mL. For inter-day and intra-day tests, the precision (RSD) for the entire validation was less than 12 %. The developed method was successfully applied to pharmacokinetic studies of norvancomycin in rats following single intravenous administration dose of 10 mg/Kg.
format Articulo
Comunicacion
author Yang, Xuezhi
Hu, Lufeng
Lin, Guanyang
Xu, Ren-ai
Zhang, Yu
Chen, Linzi
Zhu, Lihe
Zhao, Feng
Wang, Xianqin
author_facet Yang, Xuezhi
Hu, Lufeng
Lin, Guanyang
Xu, Ren-ai
Zhang, Yu
Chen, Linzi
Zhu, Lihe
Zhao, Feng
Wang, Xianqin
author_sort Yang, Xuezhi
title A simple LC-ESI-MS method for the determination of norvancomycin in rat plasma and application to pharmacokinetic study
title_short A simple LC-ESI-MS method for the determination of norvancomycin in rat plasma and application to pharmacokinetic study
title_full A simple LC-ESI-MS method for the determination of norvancomycin in rat plasma and application to pharmacokinetic study
title_fullStr A simple LC-ESI-MS method for the determination of norvancomycin in rat plasma and application to pharmacokinetic study
title_full_unstemmed A simple LC-ESI-MS method for the determination of norvancomycin in rat plasma and application to pharmacokinetic study
title_sort simple lc-esi-ms method for the determination of norvancomycin in rat plasma and application to pharmacokinetic study
publishDate 2010
url http://sedici.unlp.edu.ar/handle/10915/8024
http://www.latamjpharm.org/resumenes/29/6/LAJOP_29_6_2_9.pdf
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