Development and validation of an analytical method for the simultaneous determination of benzoylecgonine and ecgonine methyl ester in human urine by gas chromatography/mass spectrometry

A simple and rapid procedure has been developed and validated for the simultaneous determination of benzoylecgonine and ecgonine methyl ester in human urine samples. After extraction and sample cleanup by solid-phase extraction (SPE), the extracts were derivatized with 50 uL of MTBSTFA and analyzed...

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Detalles Bibliográficos
Autores principales: Montes de Oca Porto, Rodny, Correa Vidal, Teresa, Martínez Brito, Dayamín, González Hernández, Sandy, Terrero, Olga L., Granda Fraga, Mario
Formato: Articulo Comunicacion
Lenguaje:Inglés
Publicado: 2010
Materias:
Farmacia
benzoylecgonine; ecgonine methyl ester; doping control; gas chromatography/mass spectrometry
Medicamentos
Doping en los deportes
Farmacología
Bioquímica
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/7984
http://www.latamjpharm.org/resumenes/29/5/LAJOP_29_5_2_2.pdf
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-7984
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Farmacia
benzoylecgonine; ecgonine methyl ester; doping control; gas chromatography/mass spectrometry
Medicamentos
Doping en los deportes
Farmacología
Bioquímica
spellingShingle Farmacia
benzoylecgonine; ecgonine methyl ester; doping control; gas chromatography/mass spectrometry
Medicamentos
Doping en los deportes
Farmacología
Bioquímica
Montes de Oca Porto, Rodny
Correa Vidal, Teresa
Martínez Brito, Dayamín
González Hernández, Sandy
Terrero, Olga L.
Granda Fraga, Mario
Development and validation of an analytical method for the simultaneous determination of benzoylecgonine and ecgonine methyl ester in human urine by gas chromatography/mass spectrometry
topic_facet Farmacia
benzoylecgonine; ecgonine methyl ester; doping control; gas chromatography/mass spectrometry
Medicamentos
Doping en los deportes
Farmacología
Bioquímica
description A simple and rapid procedure has been developed and validated for the simultaneous determination of benzoylecgonine and ecgonine methyl ester in human urine samples. After extraction and sample cleanup by solid-phase extraction (SPE), the extracts were derivatized with 50 uL of MTBSTFA and analyzed by gas chromatography/mass spectrometry (GC/MS). The limit of detection (LOD) was 50 ng/mL for both metabolites. The recoveries were 81% and 75.3% for benzoylecgonine and ecgonine methyl ester, respectively. The intra-assay precision (% relative standard deviation) at two different added amounts (200 and 1000 ng/mL) in urine matrix varied from 4.8 to 8.4 and from 4.4 to 5.7, respectively. The mass spectra obtained for each compound have many diagnostics ions with relative abundance in accordance with the WADA requirements.
format Articulo
Comunicacion
author Montes de Oca Porto, Rodny
Correa Vidal, Teresa
Martínez Brito, Dayamín
González Hernández, Sandy
Terrero, Olga L.
Granda Fraga, Mario
author_facet Montes de Oca Porto, Rodny
Correa Vidal, Teresa
Martínez Brito, Dayamín
González Hernández, Sandy
Terrero, Olga L.
Granda Fraga, Mario
author_sort Montes de Oca Porto, Rodny
title Development and validation of an analytical method for the simultaneous determination of benzoylecgonine and ecgonine methyl ester in human urine by gas chromatography/mass spectrometry
title_short Development and validation of an analytical method for the simultaneous determination of benzoylecgonine and ecgonine methyl ester in human urine by gas chromatography/mass spectrometry
title_full Development and validation of an analytical method for the simultaneous determination of benzoylecgonine and ecgonine methyl ester in human urine by gas chromatography/mass spectrometry
title_fullStr Development and validation of an analytical method for the simultaneous determination of benzoylecgonine and ecgonine methyl ester in human urine by gas chromatography/mass spectrometry
title_full_unstemmed Development and validation of an analytical method for the simultaneous determination of benzoylecgonine and ecgonine methyl ester in human urine by gas chromatography/mass spectrometry
title_sort development and validation of an analytical method for the simultaneous determination of benzoylecgonine and ecgonine methyl ester in human urine by gas chromatography/mass spectrometry
publishDate 2010
url http://sedici.unlp.edu.ar/handle/10915/7984
http://www.latamjpharm.org/resumenes/29/5/LAJOP_29_5_2_2.pdf
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