Determination of tolbutamide in rabbit plasma by LC-MS/MS and its application to pharmacokinetic study
A sensitive and selective liquid chromatography-tandem mass spectrometry method (LC-MS/MS) for the determination of tolbutamide in rabbit plasma was developed and validated over the concentration range of 4-1000 ng mL-1. After addition of bupivacaine as internal standard (IS), a simplified protei...
Guardado en:
| Autores principales: | , , , , , , |
|---|---|
| Formato: | Articulo Comunicacion |
| Lenguaje: | Inglés |
| Publicado: |
2010
|
| Materias: | |
| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/7983 http://www.latamjpharm.org/resumenes/29/5/LAJOP_29_5_2_1.pdf |
| Aporte de: |
| Sumario: | A sensitive and selective liquid chromatography-tandem mass spectrometry method
(LC-MS/MS) for the determination of tolbutamide in rabbit plasma was developed and validated over the
concentration range of 4-1000 ng mL-1. After addition of bupivacaine as internal standard (IS), a simplified
protein precipitation with acetonitrile was employed for the sample preparation. Chromatographic
separation was performed by an Agilent Zorbax SB-C18 column (150 mmu2.1 mm, 3.5 um). The mobile
phase was acetonitrile-1% formic acid in water (50:50 v/v) delivered at a flow rate of 0.3 mL min-1. The
MS data acquisition was accomplished by multiple reactions monitoring (MRM) mode with positive electrospray
ionization (ESI) interface. The lower limit of quantification (LLOQ) was 4 ng mL-1. For interday
and intra-day tests, the precision (RSD) for the entire validation was less than 10%, and the accuracy
was within the 94.7% to 105.6% range. The validated method is successfully used to analyze the drug in
samples of rabbit plasma for pharmacokinetic study. |
|---|