Determination of tolbutamide in rabbit plasma by LC-MS/MS and its application to pharmacokinetic study

A sensitive and selective liquid chromatography-tandem mass spectrometry method (LC-MS/MS) for the determination of tolbutamide in rabbit plasma was developed and validated over the concentration range of 4-1000 ng mL-1. After addition of bupivacaine as internal standard (IS), a simplified protei...

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Detalles Bibliográficos
Autores principales: Wang, Xuebao, Lin, Guanyang, Hu, Lufeng, Xu, Xuegu, Jiang, Haiyan, Pan, Yibin, Wang, Xianqin
Formato: Articulo Comunicacion
Lenguaje:Inglés
Publicado: 2010
Materias:
Farmacia
plasma; pharmacokinetics; tolbutamide
Medicamentos
Farmacología
Cromatografía líquida
Monitoreo de drogas
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/7983
http://www.latamjpharm.org/resumenes/29/5/LAJOP_29_5_2_1.pdf
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-7983
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Farmacia
plasma; pharmacokinetics; tolbutamide
Medicamentos
Farmacología
Cromatografía líquida
Monitoreo de drogas
spellingShingle Farmacia
plasma; pharmacokinetics; tolbutamide
Medicamentos
Farmacología
Cromatografía líquida
Monitoreo de drogas
Wang, Xuebao
Lin, Guanyang
Hu, Lufeng
Xu, Xuegu
Jiang, Haiyan
Pan, Yibin
Wang, Xianqin
Determination of tolbutamide in rabbit plasma by LC-MS/MS and its application to pharmacokinetic study
topic_facet Farmacia
plasma; pharmacokinetics; tolbutamide
Medicamentos
Farmacología
Cromatografía líquida
Monitoreo de drogas
description A sensitive and selective liquid chromatography-tandem mass spectrometry method (LC-MS/MS) for the determination of tolbutamide in rabbit plasma was developed and validated over the concentration range of 4-1000 ng mL-1. After addition of bupivacaine as internal standard (IS), a simplified protein precipitation with acetonitrile was employed for the sample preparation. Chromatographic separation was performed by an Agilent Zorbax SB-C18 column (150 mmu2.1 mm, 3.5 um). The mobile phase was acetonitrile-1% formic acid in water (50:50 v/v) delivered at a flow rate of 0.3 mL min-1. The MS data acquisition was accomplished by multiple reactions monitoring (MRM) mode with positive electrospray ionization (ESI) interface. The lower limit of quantification (LLOQ) was 4 ng mL-1. For interday and intra-day tests, the precision (RSD) for the entire validation was less than 10%, and the accuracy was within the 94.7% to 105.6% range. The validated method is successfully used to analyze the drug in samples of rabbit plasma for pharmacokinetic study.
format Articulo
Comunicacion
author Wang, Xuebao
Lin, Guanyang
Hu, Lufeng
Xu, Xuegu
Jiang, Haiyan
Pan, Yibin
Wang, Xianqin
author_facet Wang, Xuebao
Lin, Guanyang
Hu, Lufeng
Xu, Xuegu
Jiang, Haiyan
Pan, Yibin
Wang, Xianqin
author_sort Wang, Xuebao
title Determination of tolbutamide in rabbit plasma by LC-MS/MS and its application to pharmacokinetic study
title_short Determination of tolbutamide in rabbit plasma by LC-MS/MS and its application to pharmacokinetic study
title_full Determination of tolbutamide in rabbit plasma by LC-MS/MS and its application to pharmacokinetic study
title_fullStr Determination of tolbutamide in rabbit plasma by LC-MS/MS and its application to pharmacokinetic study
title_full_unstemmed Determination of tolbutamide in rabbit plasma by LC-MS/MS and its application to pharmacokinetic study
title_sort determination of tolbutamide in rabbit plasma by lc-ms/ms and its application to pharmacokinetic study
publishDate 2010
url http://sedici.unlp.edu.ar/handle/10915/7983
http://www.latamjpharm.org/resumenes/29/5/LAJOP_29_5_2_1.pdf
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