Analytical techniques for deoxynivalenol detection and quantification in wheat destined for the manufacture of commercial products

The concern regarding toxicity from the presence of deoxynivalenol (DON) in wheat that affects both economy and public health leads to the need to find appropriate detection methods for determining the degree of DON contamination in terms of the equipment available and the speed required for obtaini...

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Autores principales: Astoreca, Andrea Luciana, Ortega, Leonel Maximiliano, Fígoli, Cecilia Beatriz, Cardós, M., Cavaglieri, L., Bosch, María Alejandra, Alconada Magliano, Teresa María
Formato: Articulo
Lenguaje:Inglés
Publicado: 2016
Materias:
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/79428
Aporte de:
id I19-R120-10915-79428
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Química
deoxynivalenol
wheat
high-performance liquid chromatography
Fourier-transform infrared spectroscopy
lateral-flow immunochromatography
spellingShingle Química
deoxynivalenol
wheat
high-performance liquid chromatography
Fourier-transform infrared spectroscopy
lateral-flow immunochromatography
Astoreca, Andrea Luciana
Ortega, Leonel Maximiliano
Fígoli, Cecilia Beatriz
Cardós, M.
Cavaglieri, L.
Bosch, María Alejandra
Alconada Magliano, Teresa María
Analytical techniques for deoxynivalenol detection and quantification in wheat destined for the manufacture of commercial products
topic_facet Química
deoxynivalenol
wheat
high-performance liquid chromatography
Fourier-transform infrared spectroscopy
lateral-flow immunochromatography
description The concern regarding toxicity from the presence of deoxynivalenol (DON) in wheat that affects both economy and public health leads to the need to find appropriate detection methods for determining the degree of DON contamination in terms of the equipment available and the speed required for obtaining the incidence. The objective of this study was to compare the performance of two alternative analytical techniques for DON quantification for use in the food industry with a reference technique. Samples of wheat and the commercial by-products were analysed by high-performance liquid chromatography (HPLC) with an ultraviolet detector as the reference method and the results compared with those obtained from a rapid lateral-flow immunochromatographic device (Reveal Q+) and of a Fourier-transform-infrared (FTIR) spectroscopy technique. Pearson’s correlation coefficient between the HPLC and Reveal-Q+ data (0.45), although significant (P<0.0003), was lower than that obtained between HPLC and the FTIR method (0.94, P<0.0001). Both methods were considered efficient in quantifying DON levels in wheat-flour samples. This study was aimed at assisting the producers in choosing an appropriate tool for the purpose of analysis and upon consideration of the available equipment.
format Articulo
Articulo
author Astoreca, Andrea Luciana
Ortega, Leonel Maximiliano
Fígoli, Cecilia Beatriz
Cardós, M.
Cavaglieri, L.
Bosch, María Alejandra
Alconada Magliano, Teresa María
author_facet Astoreca, Andrea Luciana
Ortega, Leonel Maximiliano
Fígoli, Cecilia Beatriz
Cardós, M.
Cavaglieri, L.
Bosch, María Alejandra
Alconada Magliano, Teresa María
author_sort Astoreca, Andrea Luciana
title Analytical techniques for deoxynivalenol detection and quantification in wheat destined for the manufacture of commercial products
title_short Analytical techniques for deoxynivalenol detection and quantification in wheat destined for the manufacture of commercial products
title_full Analytical techniques for deoxynivalenol detection and quantification in wheat destined for the manufacture of commercial products
title_fullStr Analytical techniques for deoxynivalenol detection and quantification in wheat destined for the manufacture of commercial products
title_full_unstemmed Analytical techniques for deoxynivalenol detection and quantification in wheat destined for the manufacture of commercial products
title_sort analytical techniques for deoxynivalenol detection and quantification in wheat destined for the manufacture of commercial products
publishDate 2016
url http://sedici.unlp.edu.ar/handle/10915/79428
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