Gut Permeability and Glucose Absorption Are Affected at Early Stages of Graft Rejection in a Small Bowel Transplant Rat Model

Background. Intestinal transplantation (ITx) faces many challenges due to the complexity of surgery and to the multiple immunological reactions that lead to the necessity of rigorous follow-up for early detection of acute cellular rejection (ACR). Our aim was to determine the kinetics of ACR using a...

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Autores principales: Stringa, Pablo Luis, Romanin, David Emmanuel, Lausada, Natalia Raquel, Papa Gobbi, Rodrigo, Zanuzzi, Carolina Natalia, Martín, Pedro, Abate Zárate, Juan Cruz, Cabanne, Ana, Arnal, Nathalie, Vecchio Dezillio, Leandro Emmanuel, Milesi, María Verónica, Portiansky, Enrique Leo, Gondolesi, Gabriel, Rumbo, Martín
Formato: Articulo
Lenguaje:Inglés
Publicado: 2017
Materias:
Ciencias Médicas
Intestinal transplantation
acute cellular rejection
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/79408
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-79408
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Médicas
Intestinal transplantation
acute cellular rejection
spellingShingle Ciencias Médicas
Intestinal transplantation
acute cellular rejection
Stringa, Pablo Luis
Romanin, David Emmanuel
Lausada, Natalia Raquel
Papa Gobbi, Rodrigo
Zanuzzi, Carolina Natalia
Martín, Pedro
Abate Zárate, Juan Cruz
Cabanne, Ana
Arnal, Nathalie
Vecchio Dezillio, Leandro Emmanuel
Milesi, María Verónica
Portiansky, Enrique Leo
Gondolesi, Gabriel
Rumbo, Martín
Gut Permeability and Glucose Absorption Are Affected at Early Stages of Graft Rejection in a Small Bowel Transplant Rat Model
topic_facet Ciencias Médicas
Intestinal transplantation
acute cellular rejection
description Background. Intestinal transplantation (ITx) faces many challenges due to the complexity of surgery and to the multiple immunological reactions that lead to the necessity of rigorous follow-up for early detection of acute cellular rejection (ACR). Our aim was to determine the kinetics of ACR using an experimental ITx model, with emphasis in the characterization of the process using different approaches, including the use of functional assays of absorptive and barrier function.Methods. ITx in rats conducting serial sampling was performed. Clinical monitoring, graft histology, proinflammatory gene expression, and nitrosative stress determination were performed. Also, glucose absorption, barrier function using ovalbumin translocation, and contractile function were analyzed. Results. The model used reproduced the different stages of ACR. Allogeneic ITx recipients showed signs of rejection from postoperative day (POD) 5, with increasing severity until 12 POD. Histological evaluation showed mild rejection in early sampling and severe rejection at late stages, with alterations in all graft layers. IL-6, CXCL 10, IFNg, and nitrite plasmas levels showed behavior coincident with histopathology. Remarkably, allogeneic grafts showed a marked alteration of glucose absorptive capacity from POD 5 that was sustained until endpoint. Coincidently, barrier function alteration was evidenced by luminal ovalbumin translocation to serum. Contractile function was progressively impaired along ACR. Conclusions. Glucose absorption and barrier function are altered at early stages of ACR when histological alterations or gene expression changes were much subtle. This observation may provide simple evaluation tools that could be eventually translated to the clinics to contribute to early ACR diagnosis.
format Articulo
Articulo
author Stringa, Pablo Luis
Romanin, David Emmanuel
Lausada, Natalia Raquel
Papa Gobbi, Rodrigo
Zanuzzi, Carolina Natalia
Martín, Pedro
Abate Zárate, Juan Cruz
Cabanne, Ana
Arnal, Nathalie
Vecchio Dezillio, Leandro Emmanuel
Milesi, María Verónica
Portiansky, Enrique Leo
Gondolesi, Gabriel
Rumbo, Martín
author_facet Stringa, Pablo Luis
Romanin, David Emmanuel
Lausada, Natalia Raquel
Papa Gobbi, Rodrigo
Zanuzzi, Carolina Natalia
Martín, Pedro
Abate Zárate, Juan Cruz
Cabanne, Ana
Arnal, Nathalie
Vecchio Dezillio, Leandro Emmanuel
Milesi, María Verónica
Portiansky, Enrique Leo
Gondolesi, Gabriel
Rumbo, Martín
author_sort Stringa, Pablo Luis
title Gut Permeability and Glucose Absorption Are Affected at Early Stages of Graft Rejection in a Small Bowel Transplant Rat Model
title_short Gut Permeability and Glucose Absorption Are Affected at Early Stages of Graft Rejection in a Small Bowel Transplant Rat Model
title_full Gut Permeability and Glucose Absorption Are Affected at Early Stages of Graft Rejection in a Small Bowel Transplant Rat Model
title_fullStr Gut Permeability and Glucose Absorption Are Affected at Early Stages of Graft Rejection in a Small Bowel Transplant Rat Model
title_full_unstemmed Gut Permeability and Glucose Absorption Are Affected at Early Stages of Graft Rejection in a Small Bowel Transplant Rat Model
title_sort gut permeability and glucose absorption are affected at early stages of graft rejection in a small bowel transplant rat model
publishDate 2017
url http://sedici.unlp.edu.ar/handle/10915/79408
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