HPLC analysis of bioactive steroids from the roots of Withania somnifera (L.) Dunal (Ashwagandha)
A simple procedure was described for the determination of Withanolide A and Withanolide B in Ashwagandha root by HPLC. Separation was achieved on a Spherisorb C18 column with Water: Acetonitrile in the gradient elution. Detection was accomplished with UV detection at λ = 216 nm. Using this method, t...
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| Autores principales: | , , |
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| Formato: | Articulo Comunicacion |
| Lenguaje: | Inglés |
| Publicado: |
2010
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| Materias: | |
| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/7935 http://www.latamjpharm.org/resumenes/29/3/LAJOP_29_3_2_5.pdf |
| Aporte de: |
| Sumario: | A simple procedure was described for the determination of Withanolide A and Withanolide B in Ashwagandha root by HPLC. Separation was achieved on a Spherisorb C18 column with Water: Acetonitrile in the gradient elution. Detection was accomplished with UV detection at λ = 216 nm. Using this method, the bioactive steroid in Withania somnifera was determined. Significant variations in the contents of Withanolide A and Withanolide B in Ashwagandha root growing in different locations were also observed. |
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