HPLC analysis of bioactive steroids from the roots of Withania somnifera (L.) Dunal (Ashwagandha)

A simple procedure was described for the determination of Withanolide A and Withanolide B in Ashwagandha root by HPLC. Separation was achieved on a Spherisorb C18 column with Water: Acetonitrile in the gradient elution. Detection was accomplished with UV detection at λ = 216 nm. Using this method, t...

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Detalles Bibliográficos
Autores principales: Upadhyaya, Sunil Datt, Nayak, Preeti Sagar, Upadhyaya, Anubha
Formato: Articulo Comunicacion
Lenguaje:Inglés
Publicado: 2010
Materias:
Farmacia
Farmacología
Ashwagandha; bioactive steroid; HPLC; withania somnifera; withanolide A; withanolide B
Química de los esteroides
Esteroides
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/7935
http://www.latamjpharm.org/resumenes/29/3/LAJOP_29_3_2_5.pdf
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-7935
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Farmacia
Farmacología
Ashwagandha; bioactive steroid; HPLC; withania somnifera; withanolide A; withanolide B
Química de los esteroides
Esteroides
spellingShingle Farmacia
Farmacología
Ashwagandha; bioactive steroid; HPLC; withania somnifera; withanolide A; withanolide B
Química de los esteroides
Esteroides
Upadhyaya, Sunil Datt
Nayak, Preeti Sagar
Upadhyaya, Anubha
HPLC analysis of bioactive steroids from the roots of Withania somnifera (L.) Dunal (Ashwagandha)
topic_facet Farmacia
Farmacología
Ashwagandha; bioactive steroid; HPLC; withania somnifera; withanolide A; withanolide B
Química de los esteroides
Esteroides
description A simple procedure was described for the determination of Withanolide A and Withanolide B in Ashwagandha root by HPLC. Separation was achieved on a Spherisorb C18 column with Water: Acetonitrile in the gradient elution. Detection was accomplished with UV detection at λ = 216 nm. Using this method, the bioactive steroid in Withania somnifera was determined. Significant variations in the contents of Withanolide A and Withanolide B in Ashwagandha root growing in different locations were also observed.
format Articulo
Comunicacion
author Upadhyaya, Sunil Datt
Nayak, Preeti Sagar
Upadhyaya, Anubha
author_facet Upadhyaya, Sunil Datt
Nayak, Preeti Sagar
Upadhyaya, Anubha
author_sort Upadhyaya, Sunil Datt
title HPLC analysis of bioactive steroids from the roots of Withania somnifera (L.) Dunal (Ashwagandha)
title_short HPLC analysis of bioactive steroids from the roots of Withania somnifera (L.) Dunal (Ashwagandha)
title_full HPLC analysis of bioactive steroids from the roots of Withania somnifera (L.) Dunal (Ashwagandha)
title_fullStr HPLC analysis of bioactive steroids from the roots of Withania somnifera (L.) Dunal (Ashwagandha)
title_full_unstemmed HPLC analysis of bioactive steroids from the roots of Withania somnifera (L.) Dunal (Ashwagandha)
title_sort hplc analysis of bioactive steroids from the roots of withania somnifera (l.) dunal (ashwagandha)
publishDate 2010
url http://sedici.unlp.edu.ar/handle/10915/7935
http://www.latamjpharm.org/resumenes/29/3/LAJOP_29_3_2_5.pdf
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AT nayakpreetisagar hplcanalysisofbioactivesteroidsfromtherootsofwithaniasomniferaldunalashwagandha
AT upadhyayaanubha hplcanalysisofbioactivesteroidsfromtherootsofwithaniasomniferaldunalashwagandha
bdutipo_str Repositorios
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