Modifications of <i>Bordetella bronchiseptica</i> core lipopolysaccharide influence immune response without affecting protective activity

Bordetella bronchiseptica produces respiratory disease primarily in mammals including humans. Although a considerably amount of research has been generated regarding lipopolysaccharide (LPS) role during infection and stimulating innate and adaptive immune response, mechanisms involved in LPS synthes...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Sisti, Federico, Fernández, Julieta, Cordero, Andrés Ignacio, Casabuono, Adriana, Couto, Alicia, Hozbor, Daniela Flavia
Formato: Articulo Comunicacion
Lenguaje:Inglés
Publicado: 2016
Materias:
Ciencias Exactas
Bordetella bronchiseptica
Lipopolisacáridos
Glicosiltransferasas
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/79191
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-79191
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
Bordetella bronchiseptica
Lipopolisacáridos
Glicosiltransferasas
spellingShingle Ciencias Exactas
Bordetella bronchiseptica
Lipopolisacáridos
Glicosiltransferasas
Sisti, Federico
Fernández, Julieta
Cordero, Andrés Ignacio
Casabuono, Adriana
Couto, Alicia
Hozbor, Daniela Flavia
Modifications of <i>Bordetella bronchiseptica</i> core lipopolysaccharide influence immune response without affecting protective activity
topic_facet Ciencias Exactas
Bordetella bronchiseptica
Lipopolisacáridos
Glicosiltransferasas
description Bordetella bronchiseptica produces respiratory disease primarily in mammals including humans. Although a considerably amount of research has been generated regarding lipopolysaccharide (LPS) role during infection and stimulating innate and adaptive immune response, mechanisms involved in LPS synthesis are still unknown. In this context we searched in B. bronchiseptica genome for putative glycosyltransferases. We found possible genes codifying for enzymes involved in sugar substitution of the LPS structure. We decided to analyse BB3394 to BB3400 genes, closed to a previously described LPS biosynthetic locus in B. pertussis. Particularly, conservation of BB3394 in sequenced B. bronchiseptica genomes suggests the importance of this gene for bacteria normal physiology. Deletion of BB3394 abolished resistance to naive serum as described for other LPS mutants. When purified LPS was analyzed, differences in the LPS core structure were found. Particularly, a GalNA branched sugar substitution in the core was absent in the LPS obtained from BB3394 deletion mutant. Absence of GalNA in core LPS alters immune response in vivo but is able to induce protective response against B. bronchiseptica infection.
format Articulo
Comunicacion
author Sisti, Federico
Fernández, Julieta
Cordero, Andrés Ignacio
Casabuono, Adriana
Couto, Alicia
Hozbor, Daniela Flavia
author_facet Sisti, Federico
Fernández, Julieta
Cordero, Andrés Ignacio
Casabuono, Adriana
Couto, Alicia
Hozbor, Daniela Flavia
author_sort Sisti, Federico
title Modifications of <i>Bordetella bronchiseptica</i> core lipopolysaccharide influence immune response without affecting protective activity
title_short Modifications of <i>Bordetella bronchiseptica</i> core lipopolysaccharide influence immune response without affecting protective activity
title_full Modifications of <i>Bordetella bronchiseptica</i> core lipopolysaccharide influence immune response without affecting protective activity
title_fullStr Modifications of <i>Bordetella bronchiseptica</i> core lipopolysaccharide influence immune response without affecting protective activity
title_full_unstemmed Modifications of <i>Bordetella bronchiseptica</i> core lipopolysaccharide influence immune response without affecting protective activity
title_sort modifications of <i>bordetella bronchiseptica</i> core lipopolysaccharide influence immune response without affecting protective activity
publishDate 2016
url http://sedici.unlp.edu.ar/handle/10915/79191
work_keys_str_mv AT sistifederico modificationsofibordetellabronchisepticaicorelipopolysaccharideinfluenceimmuneresponsewithoutaffectingprotectiveactivity
AT fernandezjulieta modificationsofibordetellabronchisepticaicorelipopolysaccharideinfluenceimmuneresponsewithoutaffectingprotectiveactivity
AT corderoandresignacio modificationsofibordetellabronchisepticaicorelipopolysaccharideinfluenceimmuneresponsewithoutaffectingprotectiveactivity
AT casabuonoadriana modificationsofibordetellabronchisepticaicorelipopolysaccharideinfluenceimmuneresponsewithoutaffectingprotectiveactivity
AT coutoalicia modificationsofibordetellabronchisepticaicorelipopolysaccharideinfluenceimmuneresponsewithoutaffectingprotectiveactivity
AT hozbordanielaflavia modificationsofibordetellabronchisepticaicorelipopolysaccharideinfluenceimmuneresponsewithoutaffectingprotectiveactivity
bdutipo_str Repositorios
_version_ 1764820487302545411

Ejemplares similares