Permeation of large antineoplastics into wild strain of Saccharomyces cerevisiae

he Ames Test approach. However the cell permeability of yeast has been considered a limitant factor to this kind of assay and many researchers have been introducing genetic modifications into wild strains to improve the sensitivity to chemical compounds. In our study, we used Saccharomyces cerevis...

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Detalles Bibliográficos
Autores principales: Kikuchi, Irene S., Pinto, Terezinha J. A.
Formato: Articulo Comunicacion
Lenguaje:Inglés
Publicado: 2010
Materias:
Farmacia
antineoplastics; cell permeability; cytotoxicity; yeast
Farmacología
Test de citotoxicidad
Permeabilidad de la membrana celular
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/7905
http://www.latamjpharm.org/resumenes/29/2/LAJOP_29_2_2_2.pdf
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-7905
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Farmacia
antineoplastics; cell permeability; cytotoxicity; yeast
Farmacología
Test de citotoxicidad
Permeabilidad de la membrana celular
spellingShingle Farmacia
antineoplastics; cell permeability; cytotoxicity; yeast
Farmacología
Test de citotoxicidad
Permeabilidad de la membrana celular
Kikuchi, Irene S.
Pinto, Terezinha J. A.
Permeation of large antineoplastics into wild strain of Saccharomyces cerevisiae
topic_facet Farmacia
antineoplastics; cell permeability; cytotoxicity; yeast
Farmacología
Test de citotoxicidad
Permeabilidad de la membrana celular
description he Ames Test approach. However the cell permeability of yeast has been considered a limitant factor to this kind of assay and many researchers have been introducing genetic modifications into wild strains to improve the sensitivity to chemical compounds. In our study, we used Saccharomyces cerevisiae ATCC 9763, well known and very common strain in antibiotic assays, and we evaluated the cytotoxicity of some antineoplastic agents (etoposide, epirubicin, carboplatin, cisplatin and mitoxantrone). Each culture was observed under the light of microscope and photographed. Neither genetic modification nor addition of permeation inducers, as dimethylsulfoxide (DMSO), were introduced during the assays and the cells presented good sensitivity to those compounds, demonstrating that other potential strains and characteristics of cells should be reconsidered to improve these assays apart from the cellular permeability.
format Articulo
Comunicacion
author Kikuchi, Irene S.
Pinto, Terezinha J. A.
author_facet Kikuchi, Irene S.
Pinto, Terezinha J. A.
author_sort Kikuchi, Irene S.
title Permeation of large antineoplastics into wild strain of Saccharomyces cerevisiae
title_short Permeation of large antineoplastics into wild strain of Saccharomyces cerevisiae
title_full Permeation of large antineoplastics into wild strain of Saccharomyces cerevisiae
title_fullStr Permeation of large antineoplastics into wild strain of Saccharomyces cerevisiae
title_full_unstemmed Permeation of large antineoplastics into wild strain of Saccharomyces cerevisiae
title_sort permeation of large antineoplastics into wild strain of saccharomyces cerevisiae
publishDate 2010
url http://sedici.unlp.edu.ar/handle/10915/7905
http://www.latamjpharm.org/resumenes/29/2/LAJOP_29_2_2_2.pdf
work_keys_str_mv AT kikuchiirenes permeationoflargeantineoplasticsintowildstrainofsaccharomycescerevisiae
AT pintoterezinhaja permeationoflargeantineoplasticsintowildstrainofsaccharomycescerevisiae
bdutipo_str Repositorios
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