Comparison between UV spectrophotometric and capillary electrophoresis methods for determination of rabeprazole sodium in pharmaceutical formulations
Rabeprazole sodium (RAB) is an anti-secretory agent which inhibits the enzyme H<sup>+</sup> /K<sup>+</sup> ATPase, present in the stomach parietal cells. The aim of this work is to develop and validate a simple and fast ultraviolet spectrophotometric method (UV) for quantific...
Guardado en:
| Autores principales: | , , , |
|---|---|
| Formato: | Articulo Comunicacion |
| Lenguaje: | Inglés |
| Publicado: |
2010
|
| Materias: | |
| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/7885 http://www.latamjpharm.org/trabajos/29/1/LAJOP_29_1_2_7_3KLE1K4TXH.pdf |
| Aporte de: |
| Sumario: | Rabeprazole sodium (RAB) is an anti-secretory agent which inhibits the enzyme H<sup>+</sup> /K<sup>+</sup> ATPase, present in the stomach parietal cells. The aim of this work is to develop and validate a simple and fast ultraviolet spectrophotometric method (UV) for quantification of RAB in pharmaceutical formulation and compare it with a capillary electrophoresis (CE) one, previously validated. The UV technique was applied using water (pH 10.0) as diluent and the determinations were made at λ = 291 nm. The method showed good linearity (r = 0.9997) in the concentration range of 6.0 to 18.0 μg ml<sup>-1</sup> .(The intra- and interday precision data demonstrated the method has good repeatability (RSD = 0.52 and 0.82, respectively . Accuracy and specificity were also evaluated and results were satisfactory. The detection and quantitation limits were 0.32 and 0.95 μg ml<sup>-1</sup> , respectively. Both methods demonstrated to be adequate for the intended purpose. |
|---|