Comparison between UV spectrophotometric and capillary electrophoresis methods for determination of rabeprazole sodium in pharmaceutical formulations

Rabeprazole sodium (RAB) is an anti-secretory agent which inhibits the enzyme H<sup>+</sup> /K<sup>+</sup> ATPase, present in the stomach parietal cells. The aim of this work is to develop and validate a simple and fast ultraviolet spectrophotometric method (UV) for quantific...

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Autores principales: Garcia, Cássia V., Mendez, Andreas L., Steppe, Martin, Schapoval, Elfrides E. S.
Formato: Articulo Comunicacion
Lenguaje:Inglés
Publicado: 2010
Materias:
Farmacia
Control de calidad
Farmacología
Espectrofotometría
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/7885
http://www.latamjpharm.org/trabajos/29/1/LAJOP_29_1_2_7_3KLE1K4TXH.pdf
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-7885
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Farmacia
Control de calidad
Farmacología
Espectrofotometría
spellingShingle Farmacia
Control de calidad
Farmacología
Espectrofotometría
Garcia, Cássia V.
Mendez, Andreas L.
Steppe, Martin
Schapoval, Elfrides E. S.
Comparison between UV spectrophotometric and capillary electrophoresis methods for determination of rabeprazole sodium in pharmaceutical formulations
topic_facet Farmacia
Control de calidad
Farmacología
Espectrofotometría
description Rabeprazole sodium (RAB) is an anti-secretory agent which inhibits the enzyme H<sup>+</sup> /K<sup>+</sup> ATPase, present in the stomach parietal cells. The aim of this work is to develop and validate a simple and fast ultraviolet spectrophotometric method (UV) for quantification of RAB in pharmaceutical formulation and compare it with a capillary electrophoresis (CE) one, previously validated. The UV technique was applied using water (pH 10.0) as diluent and the determinations were made at λ = 291 nm. The method showed good linearity (r = 0.9997) in the concentration range of 6.0 to 18.0 μg ml<sup>-1</sup> .(The intra- and interday precision data demonstrated the method has good repeatability (RSD = 0.52 and 0.82, respectively . Accuracy and specificity were also evaluated and results were satisfactory. The detection and quantitation limits were 0.32 and 0.95 μg ml<sup>-1</sup> , respectively. Both methods demonstrated to be adequate for the intended purpose.
format Articulo
Comunicacion
author Garcia, Cássia V.
Mendez, Andreas L.
Steppe, Martin
Schapoval, Elfrides E. S.
author_facet Garcia, Cássia V.
Mendez, Andreas L.
Steppe, Martin
Schapoval, Elfrides E. S.
author_sort Garcia, Cássia V.
title Comparison between UV spectrophotometric and capillary electrophoresis methods for determination of rabeprazole sodium in pharmaceutical formulations
title_short Comparison between UV spectrophotometric and capillary electrophoresis methods for determination of rabeprazole sodium in pharmaceutical formulations
title_full Comparison between UV spectrophotometric and capillary electrophoresis methods for determination of rabeprazole sodium in pharmaceutical formulations
title_fullStr Comparison between UV spectrophotometric and capillary electrophoresis methods for determination of rabeprazole sodium in pharmaceutical formulations
title_full_unstemmed Comparison between UV spectrophotometric and capillary electrophoresis methods for determination of rabeprazole sodium in pharmaceutical formulations
title_sort comparison between uv spectrophotometric and capillary electrophoresis methods for determination of rabeprazole sodium in pharmaceutical formulations
publishDate 2010
url http://sedici.unlp.edu.ar/handle/10915/7885
http://www.latamjpharm.org/trabajos/29/1/LAJOP_29_1_2_7_3KLE1K4TXH.pdf
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