Gramicidin ion channels in a lipid bilayer supported on polyelectrolyte multilayer films: an electrochemical impedance study

Supported membranes on polymer cushions are of fundamental interest as models for cell membranes. The use of polyelectrolyte multilayers (PEMs) assembled by the layer by layer (LbL) technique as supports for a bilayer allows for easy integration of the lipid bilayer on surfaces and devices and for...

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Detalles Bibliográficos
Autores principales: Diamanti, Eleftheria, Gutiérrez Pineda, Eduart Andrés, Politakos, Nikolaos, Andreozzi, Patrizia, Rodríguez Presa, María José, Knoll, Wolfgang, Azzaroni, Omar, Gervasi, Claudio Alfredo, Moya, Sergio E.
Formato: Articulo
Lenguaje:Inglés
Publicado: 2017
Materias:
Química
polyelectrolyte multilayers
lipid bilayers
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/78676
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-78676
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Química
polyelectrolyte multilayers
lipid bilayers
spellingShingle Química
polyelectrolyte multilayers
lipid bilayers
Diamanti, Eleftheria
Gutiérrez Pineda, Eduart Andrés
Politakos, Nikolaos
Andreozzi, Patrizia
Rodríguez Presa, María José
Knoll, Wolfgang
Azzaroni, Omar
Gervasi, Claudio Alfredo
Moya, Sergio E.
Gramicidin ion channels in a lipid bilayer supported on polyelectrolyte multilayer films: an electrochemical impedance study
topic_facet Química
polyelectrolyte multilayers
lipid bilayers
description Supported membranes on polymer cushions are of fundamental interest as models for cell membranes. The use of polyelectrolyte multilayers (PEMs) assembled by the layer by layer (LbL) technique as supports for a bilayer allows for easy integration of the lipid bilayer on surfaces and devices and for nanoscale tunable spacing of the lipid bilayer. Controlling ionic permeability in lipid bilayers supported on PEMs triggers potential applications in sensing and as models for transport phenomena in cell membranes. Lipid bilayers displaying gramicidin channels are fabricated on top of polyallylamine hydrochloride (PAH) and polystyrene sulfonate (PSS) multilayer films, by the assembly of vesicles of phosphatidylcholine and phosphatidylserine, 50:50 M/M, carrying gramicidin (GA). Quartz crystal microbalance with dissipation shows that the vesicles with GA fuse into a bilayer. Atomic force microscopy reveals that the presence of GA alters the bilayer topography resulting in depressions in the bilayer of around 70 nm in diameter. Electrochemical impedance spectroscopy (EIS) studies show that supported bilayers carrying GA have smaller resistances than the bilayers without GA. Lipid layers carrying GA display a higher conductance for K+ than for Na+ and are blocked in the presence of Ca2+.
format Articulo
Articulo
author Diamanti, Eleftheria
Gutiérrez Pineda, Eduart Andrés
Politakos, Nikolaos
Andreozzi, Patrizia
Rodríguez Presa, María José
Knoll, Wolfgang
Azzaroni, Omar
Gervasi, Claudio Alfredo
Moya, Sergio E.
author_facet Diamanti, Eleftheria
Gutiérrez Pineda, Eduart Andrés
Politakos, Nikolaos
Andreozzi, Patrizia
Rodríguez Presa, María José
Knoll, Wolfgang
Azzaroni, Omar
Gervasi, Claudio Alfredo
Moya, Sergio E.
author_sort Diamanti, Eleftheria
title Gramicidin ion channels in a lipid bilayer supported on polyelectrolyte multilayer films: an electrochemical impedance study
title_short Gramicidin ion channels in a lipid bilayer supported on polyelectrolyte multilayer films: an electrochemical impedance study
title_full Gramicidin ion channels in a lipid bilayer supported on polyelectrolyte multilayer films: an electrochemical impedance study
title_fullStr Gramicidin ion channels in a lipid bilayer supported on polyelectrolyte multilayer films: an electrochemical impedance study
title_full_unstemmed Gramicidin ion channels in a lipid bilayer supported on polyelectrolyte multilayer films: an electrochemical impedance study
title_sort gramicidin ion channels in a lipid bilayer supported on polyelectrolyte multilayer films: an electrochemical impedance study
publishDate 2017
url http://sedici.unlp.edu.ar/handle/10915/78676
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