Rapid determination of hydrochlorothiazide in human plasma by high performance liquid chromatography-tandem mass spectrometry

This paper describes a rapid (2.0 min) and sensitive (LLOQ 5 ng/mL) analytical method for the quantitation of hydrochlorothiazide (HCTZ) in human plasma. The method is based on High-performance Liquid chromatography-tandem mass spectrometry (LC-MS/MS) using clortalidone as internal standard (I.S.)....

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Autores principales: Sousa, Carlos E.M., Bedor, Danilo C. G., Gonçalves, Talita M., Ramos, Virna L.S., Carvalho, Andre L.M., Albuquerque, Miracy M., Santana, Davi P.
Formato: Articulo Comunicacion
Lenguaje:Inglés
Publicado: 2009
Materias:
Farmacia
hydrochlorothiazide; LC-MS/MS; pharmacokinetics
Farmacología
Cromatografía
Cromatografia líquida de alta eficiencia
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/7838
http://www.latamjpharm.org/trabajos/28/5/LAJOP_28_5_2_2_23Z390N8KH.pdf
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-7838
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Farmacia
hydrochlorothiazide; LC-MS/MS; pharmacokinetics
Farmacología
Cromatografía
Cromatografia líquida de alta eficiencia
spellingShingle Farmacia
hydrochlorothiazide; LC-MS/MS; pharmacokinetics
Farmacología
Cromatografía
Cromatografia líquida de alta eficiencia
Sousa, Carlos E.M.
Bedor, Danilo C. G.
Gonçalves, Talita M.
Ramos, Virna L.S.
Carvalho, Andre L.M.
Albuquerque, Miracy M.
Santana, Davi P.
Rapid determination of hydrochlorothiazide in human plasma by high performance liquid chromatography-tandem mass spectrometry
topic_facet Farmacia
hydrochlorothiazide; LC-MS/MS; pharmacokinetics
Farmacología
Cromatografía
Cromatografia líquida de alta eficiencia
description This paper describes a rapid (2.0 min) and sensitive (LLOQ 5 ng/mL) analytical method for the quantitation of hydrochlorothiazide (HCTZ) in human plasma. The method is based on High-performance Liquid chromatography-tandem mass spectrometry (LC-MS/MS) using clortalidone as internal standard (I.S.). Sample preparation involved liquid-liquid extraction with methyl tert-butyl ether. The chromatographic separation was achieved on a monolitic C18 (50 x 4,6 mm) reversed-phase column and a mobile phase containing acetonitrile/water (80:20 v/v, add 5% isopropyl alcohol), in isocratic conditions. The target analytes were transferred into a triple quadrupole mass spectrometer equipped with an electrospray ionization source for mass detection. The ion transitions selected for MRM detection were: m/z 296.10 > 204.85 and 337.13 > 189.77 for HCTZ and I.S., respectively. The assay was linear in the concentration range of 5-400 ng/mL. The mean recovery for HCTZ was 80.46%. Intra- and inter-day precision (Relative Standard Desviation) were < 10.3 % and <11.7%, respectively and the accuracy (Relative Error) was in the range ± 4.54%, the accuracy was evaluated by the ratio between concentration found/nominal concentration. The method was successfully applied to a single oral dose pharmacokinetics study in 26 healthy human volunteers
format Articulo
Comunicacion
author Sousa, Carlos E.M.
Bedor, Danilo C. G.
Gonçalves, Talita M.
Ramos, Virna L.S.
Carvalho, Andre L.M.
Albuquerque, Miracy M.
Santana, Davi P.
author_facet Sousa, Carlos E.M.
Bedor, Danilo C. G.
Gonçalves, Talita M.
Ramos, Virna L.S.
Carvalho, Andre L.M.
Albuquerque, Miracy M.
Santana, Davi P.
author_sort Sousa, Carlos E.M.
title Rapid determination of hydrochlorothiazide in human plasma by high performance liquid chromatography-tandem mass spectrometry
title_short Rapid determination of hydrochlorothiazide in human plasma by high performance liquid chromatography-tandem mass spectrometry
title_full Rapid determination of hydrochlorothiazide in human plasma by high performance liquid chromatography-tandem mass spectrometry
title_fullStr Rapid determination of hydrochlorothiazide in human plasma by high performance liquid chromatography-tandem mass spectrometry
title_full_unstemmed Rapid determination of hydrochlorothiazide in human plasma by high performance liquid chromatography-tandem mass spectrometry
title_sort rapid determination of hydrochlorothiazide in human plasma by high performance liquid chromatography-tandem mass spectrometry
publishDate 2009
url http://sedici.unlp.edu.ar/handle/10915/7838
http://www.latamjpharm.org/trabajos/28/5/LAJOP_28_5_2_2_23Z390N8KH.pdf
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