Molecular Genetic Profiling of Clinical and Foodborne Strains of Enterococci with High Level Resistance to Gentamicin and Vancomycin

Enterococci often acquire antimicrobial resistance through horizontal gene transfer. Relatedness between enterococci with high level resistance to gentamicin and vancomycin isolated from humans, food and hospital environment in Tandil County (Argentina) was investigated. PCR amplification for specie...

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Detalles Bibliográficos
Autores principales: Pourcel, Natalia Gisela, Sparo, Mónica Delfina, Corso, Alejandra, Delpech, Gastón, Gagetti, Paula, De Luca, María Marta, Bernstein, Judith Celina, Schell, Celia María Beatriz, Lissarrague, Sabina, Basualdo Farjat, Juan Ángel
Formato: Articulo
Lenguaje:Inglés
Publicado: 2017
Materias:
Ciencias Médicas
Enterococci
relatedness
food
humans
environment
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/78338
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-78338
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Médicas
Enterococci
relatedness
food
humans
environment
spellingShingle Ciencias Médicas
Enterococci
relatedness
food
humans
environment
Pourcel, Natalia Gisela
Sparo, Mónica Delfina
Corso, Alejandra
Delpech, Gastón
Gagetti, Paula
De Luca, María Marta
Bernstein, Judith Celina
Schell, Celia María Beatriz
Lissarrague, Sabina
Basualdo Farjat, Juan Ángel
Molecular Genetic Profiling of Clinical and Foodborne Strains of Enterococci with High Level Resistance to Gentamicin and Vancomycin
topic_facet Ciencias Médicas
Enterococci
relatedness
food
humans
environment
description Enterococci often acquire antimicrobial resistance through horizontal gene transfer. Relatedness between enterococci with high level resistance to gentamicin and vancomycin isolated from humans, food and hospital environment in Tandil County (Argentina) was investigated. PCR amplification for species determination was carried out. Resistance to seven antimicrobials was studied; virulence genes (esp, cylA), vancomycin and gentamicin resistance genes were investigated. In the isolates with high level antimicrobial resistance (gentamicin, vancomycin), pulse-field gel electrophoresis was performed. Vancomycin-resistant E. faecium (n:13) were recovered from human, food and hospital environment samples. All the isolates expressed high-level vancomycin and teicoplanin (vanA genotype), as well high-level gentamicin and streptomycin resistance. Vancomycin-resistant E. faecium were distributed among seven clonal types; esp gene was detected in clinical strains. There was no clonal relationship with food vanA E. faecium, but these strains could pose a risk in intra/inter genus transfer of vanA determinant to human-adapted strains. High-level gentamicin resistant E. faecalis (n:7) were recovered from human and food samples. Glycopeptide resistance was not observed; cylA gene was detected in most of the clinical high-level gentamicin resistant E. faecalis isolates. PFGE patterns showed four clonal types in high-level gentamicin resistant E. faecalis strains; there was demonstrated clonal relatedness between isolates from different origin. In Argentina, this is the first study showing a clonal relationship between high-level gentamicin resistant E. faecalis isolated from food and humans. These results encourage the study of dissemination of clonal complexes with mobile resistance genes.
format Articulo
Articulo
author Pourcel, Natalia Gisela
Sparo, Mónica Delfina
Corso, Alejandra
Delpech, Gastón
Gagetti, Paula
De Luca, María Marta
Bernstein, Judith Celina
Schell, Celia María Beatriz
Lissarrague, Sabina
Basualdo Farjat, Juan Ángel
author_facet Pourcel, Natalia Gisela
Sparo, Mónica Delfina
Corso, Alejandra
Delpech, Gastón
Gagetti, Paula
De Luca, María Marta
Bernstein, Judith Celina
Schell, Celia María Beatriz
Lissarrague, Sabina
Basualdo Farjat, Juan Ángel
author_sort Pourcel, Natalia Gisela
title Molecular Genetic Profiling of Clinical and Foodborne Strains of Enterococci with High Level Resistance to Gentamicin and Vancomycin
title_short Molecular Genetic Profiling of Clinical and Foodborne Strains of Enterococci with High Level Resistance to Gentamicin and Vancomycin
title_full Molecular Genetic Profiling of Clinical and Foodborne Strains of Enterococci with High Level Resistance to Gentamicin and Vancomycin
title_fullStr Molecular Genetic Profiling of Clinical and Foodborne Strains of Enterococci with High Level Resistance to Gentamicin and Vancomycin
title_full_unstemmed Molecular Genetic Profiling of Clinical and Foodborne Strains of Enterococci with High Level Resistance to Gentamicin and Vancomycin
title_sort molecular genetic profiling of clinical and foodborne strains of enterococci with high level resistance to gentamicin and vancomycin
publishDate 2017
url http://sedici.unlp.edu.ar/handle/10915/78338
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