The MicroRNA390/TAS3 Pathway Mediates Symbiotic Nodulation and Lateral Root Growth

Legume roots form two types of postembryonic organs, lateral roots and symbiotic nodules. Nodule formation is the result of the interaction of legumes with rhizobia and requires the mitotic activation and differentiation of root cells as well as an independent, but coordinated, program that allows i...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Hobecker, Karen Vanesa, Reynoso, Mauricio Alberto, Bustos-Sanmamed, Pilar, Wen, J., Kirankuma,r S. Mysore, Crespi, Martín, Blanco, Flavio Antonio, Zanetti, María Eugenia
Formato: Articulo
Lenguaje:Inglés
Publicado: 2017
Materias:
Biología
legumes
miR390
nodulation
Crecimiento
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/78172
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-78172
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Biología
legumes
miR390
nodulation
Crecimiento
spellingShingle Biología
legumes
miR390
nodulation
Crecimiento
Hobecker, Karen Vanesa
Reynoso, Mauricio Alberto
Bustos-Sanmamed, Pilar
Wen, J.
Kirankuma,r S. Mysore,
Crespi, Martín
Blanco, Flavio Antonio
Zanetti, María Eugenia
The MicroRNA390/TAS3 Pathway Mediates Symbiotic Nodulation and Lateral Root Growth
topic_facet Biología
legumes
miR390
nodulation
Crecimiento
description Legume roots form two types of postembryonic organs, lateral roots and symbiotic nodules. Nodule formation is the result of the interaction of legumes with rhizobia and requires the mitotic activation and differentiation of root cells as well as an independent, but coordinated, program that allows infection by rhizobia. MicroRNA390 (miR390) is an evolutionarily conserved microRNA that targets the Trans-Acting Short Interference RNA3 (TAS3) transcript. Cleavage of TAS3 by ARGONAUTE7 results in the production of trans-acting small interference RNAs, which target mRNAs encoding AUXIN RESPONSE FACTOR2 (ARF2), ARF3, and ARF4. Here, we show that activation of the miR390/TAS3 regulatory module by overexpression of miR390 in Medicago truncatula promotes lateral root growth but prevents nodule organogenesis, rhizobial infection, and the induction of two key nodulation genes, Nodulation Signaling Pathway1 (NSP1) and NSP2. Accordingly, inactivation of the miR390/TAS3 module, either by expression of a miR390 target mimicry construct or mutations in ARGONAUTE7, enhances nodulation and rhizobial infection, alters the spatial distribution of the nodules, and increases the percentage of nodules with multiple meristems. Our results revealed a key role of the miR390/TAS3 pathway in legumes as a modulator of lateral root organs, playing opposite roles in lateral root and nodule development.
format Articulo
Articulo
author Hobecker, Karen Vanesa
Reynoso, Mauricio Alberto
Bustos-Sanmamed, Pilar
Wen, J.
Kirankuma,r S. Mysore,
Crespi, Martín
Blanco, Flavio Antonio
Zanetti, María Eugenia
author_facet Hobecker, Karen Vanesa
Reynoso, Mauricio Alberto
Bustos-Sanmamed, Pilar
Wen, J.
Kirankuma,r S. Mysore,
Crespi, Martín
Blanco, Flavio Antonio
Zanetti, María Eugenia
author_sort Hobecker, Karen Vanesa
title The MicroRNA390/TAS3 Pathway Mediates Symbiotic Nodulation and Lateral Root Growth
title_short The MicroRNA390/TAS3 Pathway Mediates Symbiotic Nodulation and Lateral Root Growth
title_full The MicroRNA390/TAS3 Pathway Mediates Symbiotic Nodulation and Lateral Root Growth
title_fullStr The MicroRNA390/TAS3 Pathway Mediates Symbiotic Nodulation and Lateral Root Growth
title_full_unstemmed The MicroRNA390/TAS3 Pathway Mediates Symbiotic Nodulation and Lateral Root Growth
title_sort microrna390/tas3 pathway mediates symbiotic nodulation and lateral root growth
publishDate 2017
url http://sedici.unlp.edu.ar/handle/10915/78172
work_keys_str_mv AT hobeckerkarenvanesa themicrorna390tas3pathwaymediatessymbioticnodulationandlateralrootgrowth
AT reynosomauricioalberto themicrorna390tas3pathwaymediatessymbioticnodulationandlateralrootgrowth
AT bustossanmamedpilar themicrorna390tas3pathwaymediatessymbioticnodulationandlateralrootgrowth
AT wenj themicrorna390tas3pathwaymediatessymbioticnodulationandlateralrootgrowth
AT kirankumarsmysore themicrorna390tas3pathwaymediatessymbioticnodulationandlateralrootgrowth
AT crespimartin themicrorna390tas3pathwaymediatessymbioticnodulationandlateralrootgrowth
AT blancoflavioantonio themicrorna390tas3pathwaymediatessymbioticnodulationandlateralrootgrowth
AT zanettimariaeugenia themicrorna390tas3pathwaymediatessymbioticnodulationandlateralrootgrowth
AT hobeckerkarenvanesa microrna390tas3pathwaymediatessymbioticnodulationandlateralrootgrowth
AT reynosomauricioalberto microrna390tas3pathwaymediatessymbioticnodulationandlateralrootgrowth
AT bustossanmamedpilar microrna390tas3pathwaymediatessymbioticnodulationandlateralrootgrowth
AT wenj microrna390tas3pathwaymediatessymbioticnodulationandlateralrootgrowth
AT kirankumarsmysore microrna390tas3pathwaymediatessymbioticnodulationandlateralrootgrowth
AT crespimartin microrna390tas3pathwaymediatessymbioticnodulationandlateralrootgrowth
AT blancoflavioantonio microrna390tas3pathwaymediatessymbioticnodulationandlateralrootgrowth
AT zanettimariaeugenia microrna390tas3pathwaymediatessymbioticnodulationandlateralrootgrowth
bdutipo_str Repositorios
_version_ 1764820485502140417

Ejemplares similares