Narrowing the Knowledge Gaps on the Duration of Transferred Protective Immunity and on Vaccination Frequency

Maternal safety through pertussis vaccination and subsequent maternal–fetal-antibody transfer are well documented, but information on infant protection from pertussis by such antibodies and by subsequent vaccinations is scarce. Since mice are used extensively for maternal-vaccination studies, we ado...

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Autores principales: Gaillard, María Emilia, Bottero, Daniela, Zurita, María Eugenia, Carriquiriborde, Francisco, Aispuro, Pablo Martín, Bartel, Erika, Sabater Martínez, David, Bravo, María Sol, Castuma, Cecilia, Hozbor, Daniela Flavia
Formato: Articulo
Lenguaje:Inglés
Publicado: 2017
Materias:
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/78156
Aporte de:
id I19-R120-10915-78156
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Biología
Bordetella pertussis
pregnancy immunization
acellular vaccine
protection
spellingShingle Biología
Bordetella pertussis
pregnancy immunization
acellular vaccine
protection
Gaillard, María Emilia
Bottero, Daniela
Zurita, María Eugenia
Carriquiriborde, Francisco
Aispuro, Pablo Martín
Bartel, Erika
Sabater Martínez, David
Bravo, María Sol
Castuma, Cecilia
Hozbor, Daniela Flavia
Narrowing the Knowledge Gaps on the Duration of Transferred Protective Immunity and on Vaccination Frequency
topic_facet Biología
Bordetella pertussis
pregnancy immunization
acellular vaccine
protection
description Maternal safety through pertussis vaccination and subsequent maternal–fetal-antibody transfer are well documented, but information on infant protection from pertussis by such antibodies and by subsequent vaccinations is scarce. Since mice are used extensively for maternal-vaccination studies, we adopted that model to narrow those gaps in our understanding of maternal pertussis immunization. Accordingly, we vaccinated female mice with commercial acellular pertussis (aP) vaccine and measured offspring protection against Bordetella pertussis challenge and specific-antibody levels with or without revaccination. Maternal immunization protected the offspring against pertussis, with that immune protection transferred to the offspring lasting for several weeks, as evidenced by a reduction (4–5 logs, p < 0.001) in the colony-forming-units recovered from the lungs of 16-week-old offspring. Moreover, maternal-vaccination-acquired immunity from the first pregnancy still conferred protection to offspring up to the fourth pregnancy. Under the conditions of our experimental protocol, protection to offspring from the aP-induced immunity is transferred both transplacentally and through breastfeeding. Adoptive-transfer experiments demonstrated that transferred antibodies were more responsible for the protection detected in offspring than transferred whole spleen cells. In contrast to reported findings, the protection transferred was not lost after the vaccination of infant mice with the same or other vaccine preparations, and conversely, the immunity transferred from mothers did not interfere with the protection conferred by infant vaccination with the same or different vaccines. These results indicated that aP-vaccine immunization of pregnant female mice conferred protective immunity that is transferred both transplacentally and via offspring breastfeeding without compromising the protection boostered by subsequent infant vaccination. These results—though admittedly not necessarily immediately extrapolatable to humans—nevertheless enabled us to test hypotheses under controlled conditions through detailed sampling and data collection. These findings will hopefully refine hypotheses that can then be validated in subsequent human studies.
format Articulo
Articulo
author Gaillard, María Emilia
Bottero, Daniela
Zurita, María Eugenia
Carriquiriborde, Francisco
Aispuro, Pablo Martín
Bartel, Erika
Sabater Martínez, David
Bravo, María Sol
Castuma, Cecilia
Hozbor, Daniela Flavia
author_facet Gaillard, María Emilia
Bottero, Daniela
Zurita, María Eugenia
Carriquiriborde, Francisco
Aispuro, Pablo Martín
Bartel, Erika
Sabater Martínez, David
Bravo, María Sol
Castuma, Cecilia
Hozbor, Daniela Flavia
author_sort Gaillard, María Emilia
title Narrowing the Knowledge Gaps on the Duration of Transferred Protective Immunity and on Vaccination Frequency
title_short Narrowing the Knowledge Gaps on the Duration of Transferred Protective Immunity and on Vaccination Frequency
title_full Narrowing the Knowledge Gaps on the Duration of Transferred Protective Immunity and on Vaccination Frequency
title_fullStr Narrowing the Knowledge Gaps on the Duration of Transferred Protective Immunity and on Vaccination Frequency
title_full_unstemmed Narrowing the Knowledge Gaps on the Duration of Transferred Protective Immunity and on Vaccination Frequency
title_sort narrowing the knowledge gaps on the duration of transferred protective immunity and on vaccination frequency
publishDate 2017
url http://sedici.unlp.edu.ar/handle/10915/78156
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