Crude flavonoids from Carya cathayensis Sargent inhibited HeLa cells proliferation through induction of apoptosis and cell cycle arrest

The aim of this study was to investigate the anticancer properties of crude flavonoids from Carya cathayensis Sargent bark (CCS-F), including the inhibitory effect of CCS-F on HeLa cells and the apoptosis-inducing capacity in vitro. In our results, treatment of HeLa cells with CCS-F resulted in the...

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Detalles Bibliográficos
Autores principales: Lu, Wenzong, Yang, Yali, Li, Qingwang, Liu, Fuzhu
Formato: Articulo
Lenguaje:Inglés
Publicado: 2009
Materias:
Farmacia
Plantas medicinales
Carya cathayensis sargent; flavonoids; anticancer; apoptosis; HeLa
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/7802
http://www.latamjpharm.org/trabajos/28/4/LAJOP_28_4_1_14_9VU5452UG0.pdf
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-7802
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Farmacia
Plantas medicinales
Carya cathayensis sargent; flavonoids; anticancer; apoptosis; HeLa
spellingShingle Farmacia
Plantas medicinales
Carya cathayensis sargent; flavonoids; anticancer; apoptosis; HeLa
Lu, Wenzong
Yang, Yali
Li, Qingwang
Liu, Fuzhu
Crude flavonoids from Carya cathayensis Sargent inhibited HeLa cells proliferation through induction of apoptosis and cell cycle arrest
topic_facet Farmacia
Plantas medicinales
Carya cathayensis sargent; flavonoids; anticancer; apoptosis; HeLa
description The aim of this study was to investigate the anticancer properties of crude flavonoids from Carya cathayensis Sargent bark (CCS-F), including the inhibitory effect of CCS-F on HeLa cells and the apoptosis-inducing capacity in vitro. In our results, treatment of HeLa cells with CCS-F resulted in the growth inhibition effect, and the IC50 was 95 μg/ml. Detection of apoptosis was performed by acridine orange / ethidium bromide (AO/EB) and Tdt-mediated dUTP nick end labeling (TUNEL) staining assays, which showed more apoptosis cells in CCS-F treatment group than the control group. Furthermore, CCSF (100 μg/ml) could arrest the cells in G0/G1 phase. Meanwhile, the expression of Bax was increased in the cells treated with CCS-F (100 μg/ml), with an increase in the activity of caspase-3, while Bcl-2 expression was decreased compared to the control cells. It demonstrated that CCS-F had antiproliferative activity in HeLa cells and might be a potential anticancer drug.
format Articulo
Articulo
author Lu, Wenzong
Yang, Yali
Li, Qingwang
Liu, Fuzhu
author_facet Lu, Wenzong
Yang, Yali
Li, Qingwang
Liu, Fuzhu
author_sort Lu, Wenzong
title Crude flavonoids from Carya cathayensis Sargent inhibited HeLa cells proliferation through induction of apoptosis and cell cycle arrest
title_short Crude flavonoids from Carya cathayensis Sargent inhibited HeLa cells proliferation through induction of apoptosis and cell cycle arrest
title_full Crude flavonoids from Carya cathayensis Sargent inhibited HeLa cells proliferation through induction of apoptosis and cell cycle arrest
title_fullStr Crude flavonoids from Carya cathayensis Sargent inhibited HeLa cells proliferation through induction of apoptosis and cell cycle arrest
title_full_unstemmed Crude flavonoids from Carya cathayensis Sargent inhibited HeLa cells proliferation through induction of apoptosis and cell cycle arrest
title_sort crude flavonoids from carya cathayensis sargent inhibited hela cells proliferation through induction of apoptosis and cell cycle arrest
publishDate 2009
url http://sedici.unlp.edu.ar/handle/10915/7802
http://www.latamjpharm.org/trabajos/28/4/LAJOP_28_4_1_14_9VU5452UG0.pdf
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AT yangyali crudeflavonoidsfromcaryacathayensissargentinhibitedhelacellsproliferationthroughinductionofapoptosisandcellcyclearrest
AT liqingwang crudeflavonoidsfromcaryacathayensissargentinhibitedhelacellsproliferationthroughinductionofapoptosisandcellcyclearrest
AT liufuzhu crudeflavonoidsfromcaryacathayensissargentinhibitedhelacellsproliferationthroughinductionofapoptosisandcellcyclearrest
bdutipo_str Repositorios
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