Enzyme-Linked Immunosorbent Assays Using the Recombinant gp51 and p24 of Bovine Leukemia Virus for Immunodetection of the Disease

Enzyme-linked immunosorbent assay (ELISA) is often used to test bovine leukemia virus (BLV) infection. However, commercially available kits test in South America detect only antibodies against the gp51 protein. With the aim to improve the sensitivity of the test, we developed here a two-step indirec...

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Detalles Bibliográficos
Autores principales: Larsen, Alejandra Edith, Corva, Santiago Gerardo, Panei, Carlos Javier, Geisler, Christoph, Mórtola, Eduardo Carlos
Formato: Articulo
Lenguaje:Inglés
Publicado: 2017
Materias:
Ciencias Veterinarias
bovine leukemia virus
indirect dual ELISA
recombinant antigens
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/77892
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-77892
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Veterinarias
bovine leukemia virus
indirect dual ELISA
recombinant antigens
spellingShingle Ciencias Veterinarias
bovine leukemia virus
indirect dual ELISA
recombinant antigens
Larsen, Alejandra Edith
Corva, Santiago Gerardo
Panei, Carlos Javier
Geisler, Christoph
Mórtola, Eduardo Carlos
Enzyme-Linked Immunosorbent Assays Using the Recombinant gp51 and p24 of Bovine Leukemia Virus for Immunodetection of the Disease
topic_facet Ciencias Veterinarias
bovine leukemia virus
indirect dual ELISA
recombinant antigens
description Enzyme-linked immunosorbent assay (ELISA) is often used to test bovine leukemia virus (BLV) infection. However, commercially available kits test in South America detect only antibodies against the gp51 protein. With the aim to improve the sensitivity of the test, we developed here a two-step indirect dual ELISA test that included both proteins p24 and gp51, expressed and produced in E. coli and baculovirus expression system respectively. Two hundred ten BLV sera, stated as double positive or double negative by the combination of commercial agar gel immunodiffusion (AGID) assay and a gp51- ELISA test, were tested with our in house dual rp24/rgp51 ELISA. Firstly, we checked the purified, optimized and standardized proteins as antigen by the checkerboard technique, and set up our in house ELISA test. The concordance correlation coefficient (CCC) and coefficient of variation (CV) intraplate repeatability levels were within the values established by the international standards. The statistical analysis demonstrated the value of sera correctly ranked highest (93.48%), and for 0.3 cutoff, the sensitivity was 95.65% and the specificity 91.30%. In conclusion, the rp24/rgp51 ELISA developed and standardized here demonstrated to have good analytical characteristics to be considered for screening of BLV.
format Articulo
Articulo
author Larsen, Alejandra Edith
Corva, Santiago Gerardo
Panei, Carlos Javier
Geisler, Christoph
Mórtola, Eduardo Carlos
author_facet Larsen, Alejandra Edith
Corva, Santiago Gerardo
Panei, Carlos Javier
Geisler, Christoph
Mórtola, Eduardo Carlos
author_sort Larsen, Alejandra Edith
title Enzyme-Linked Immunosorbent Assays Using the Recombinant gp51 and p24 of Bovine Leukemia Virus for Immunodetection of the Disease
title_short Enzyme-Linked Immunosorbent Assays Using the Recombinant gp51 and p24 of Bovine Leukemia Virus for Immunodetection of the Disease
title_full Enzyme-Linked Immunosorbent Assays Using the Recombinant gp51 and p24 of Bovine Leukemia Virus for Immunodetection of the Disease
title_fullStr Enzyme-Linked Immunosorbent Assays Using the Recombinant gp51 and p24 of Bovine Leukemia Virus for Immunodetection of the Disease
title_full_unstemmed Enzyme-Linked Immunosorbent Assays Using the Recombinant gp51 and p24 of Bovine Leukemia Virus for Immunodetection of the Disease
title_sort enzyme-linked immunosorbent assays using the recombinant gp51 and p24 of bovine leukemia virus for immunodetection of the disease
publishDate 2017
url http://sedici.unlp.edu.ar/handle/10915/77892
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bdutipo_str Repositorios
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