Comparative study on the pharmacokinetics of Ginkgo biloba extract between normal and diabetic rats by HPLC-DAD

A new HPLC-DAD method has been developed and validated for the simultaneous analysis of five flavonoids (rutin, quercitrin, quercetin, kaempferol, and isorahamnetin) in rat plasma. Sample pretreatment consisted of a liquid–liquid extraction. The five flavonoids was separated on a kromasil C18 column...

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Autores principales: Tang, Daoquan, Yin, Xiaoxin, Zhang, Zunjian, Gao, Yuanyuan, Wei, Yaqin, Chen, Yonggang, Han, Lin
Formato: Articulo
Lenguaje:Inglés
Publicado: 2009
Materias:
Farmacia
Plantas medicinales
ginkgo biloba extract; high performance liquid chromatography; isorhamnetin; kaempferol; quercitrin; pharmacokinetic; quercetin; rutin
Extractos de plantas
Cromatografia líquida de alta eficiencia
Farmacocinética
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/7775
http://www.latamjpharm.org/trabajos/28/3/LAJOP_28_3_1_11_N804613NI3.pdf
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-7775
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Farmacia
Plantas medicinales
ginkgo biloba extract; high performance liquid chromatography; isorhamnetin; kaempferol; quercitrin; pharmacokinetic; quercetin; rutin
Extractos de plantas
Cromatografia líquida de alta eficiencia
Farmacocinética
spellingShingle Farmacia
Plantas medicinales
ginkgo biloba extract; high performance liquid chromatography; isorhamnetin; kaempferol; quercitrin; pharmacokinetic; quercetin; rutin
Extractos de plantas
Cromatografia líquida de alta eficiencia
Farmacocinética
Tang, Daoquan
Yin, Xiaoxin
Zhang, Zunjian
Gao, Yuanyuan
Wei, Yaqin
Chen, Yonggang
Han, Lin
Comparative study on the pharmacokinetics of Ginkgo biloba extract between normal and diabetic rats by HPLC-DAD
topic_facet Farmacia
Plantas medicinales
ginkgo biloba extract; high performance liquid chromatography; isorhamnetin; kaempferol; quercitrin; pharmacokinetic; quercetin; rutin
Extractos de plantas
Cromatografia líquida de alta eficiencia
Farmacocinética
description A new HPLC-DAD method has been developed and validated for the simultaneous analysis of five flavonoids (rutin, quercitrin, quercetin, kaempferol, and isorahamnetin) in rat plasma. Sample pretreatment consisted of a liquid–liquid extraction. The five flavonoids was separated on a kromasil C18 column and recorded at 350 nm. The greatest resolution was achieved with methanol-0.1% formic acid gradient at a flow rate of 1.0 mL min<sup>-1</sup> (The correlation coefficients for all the calibration plots (r > 0.999 . showed good linearity over the range tested. The relative standard deviation of the method was less than 7% and 10% for intra and inter-day assays respectively, and average recovery was between 77.2 and 99.2%. The detection limits of this method were between 0.006 and 0.02 μg mL<sup>-1</sup> The method has been . successfully applied to determine drug concentrations in normal and diabetic rat plasma samples and pharmacokinetic of Ginkgo biloba extract
format Articulo
Articulo
author Tang, Daoquan
Yin, Xiaoxin
Zhang, Zunjian
Gao, Yuanyuan
Wei, Yaqin
Chen, Yonggang
Han, Lin
author_facet Tang, Daoquan
Yin, Xiaoxin
Zhang, Zunjian
Gao, Yuanyuan
Wei, Yaqin
Chen, Yonggang
Han, Lin
author_sort Tang, Daoquan
title Comparative study on the pharmacokinetics of Ginkgo biloba extract between normal and diabetic rats by HPLC-DAD
title_short Comparative study on the pharmacokinetics of Ginkgo biloba extract between normal and diabetic rats by HPLC-DAD
title_full Comparative study on the pharmacokinetics of Ginkgo biloba extract between normal and diabetic rats by HPLC-DAD
title_fullStr Comparative study on the pharmacokinetics of Ginkgo biloba extract between normal and diabetic rats by HPLC-DAD
title_full_unstemmed Comparative study on the pharmacokinetics of Ginkgo biloba extract between normal and diabetic rats by HPLC-DAD
title_sort comparative study on the pharmacokinetics of ginkgo biloba extract between normal and diabetic rats by hplc-dad
publishDate 2009
url http://sedici.unlp.edu.ar/handle/10915/7775
http://www.latamjpharm.org/trabajos/28/3/LAJOP_28_3_1_11_N804613NI3.pdf
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