Screening of cytotoxic activity in hexanic and ethanolic extracts of Rollinia laurifolia

The hexanic and ethanolic extracts obtained from Rollinia laurifolia, collected in Minas Gerais State/Brazil, were screened by the brine shrimp lethality and cytotoxic assays on different cell lines, in order to identify potential sources for novel antitumoral compounds. Fifty percent of the ethanol...

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Autores principales: Cunha, M. M., Nascimento, F. C., Santos Pimento, L. P., Boaventura, M. A. D., Salas, C. E., Lopes, M. T. P.
Formato: Articulo
Lenguaje:Inglés
Publicado: 2009
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Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/7751
http://www.latamjpharm.org/trabajos/28/2/LAJOP_28_2_1_10_M2NU25E601.pdf
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Sumario:The hexanic and ethanolic extracts obtained from Rollinia laurifolia, collected in Minas Gerais State/Brazil, were screened by the brine shrimp lethality and cytotoxic assays on different cell lines, in order to identify potential sources for novel antitumoral compounds. Fifty percent of the ethanolic derived fractions (E) showed significant cytotoxic activity (IC<sub>50</sub> < 10–1 μg/mL), not related to tannins or saponins, i. e., IC<sub>50</sub> E13 on C6 = 1.54 x 10<sup>-2</sup> μg/mL, IC<sub>50</sub> E29 on L<sub>929</sub> and C<sub>6</sub> = 6.43 x 10<sup>-1</sup> μg/mL and 3.57 x 10<sup>-2</sup> μg/mL, respectively. RP-HPLC analysis led to the purification and identification of two known acetogenins, gonionenin and annonin-I, detected for the first time in Rollinia sp, and a new acetogenin, named laurifolin.