Cytotoxicity of dehydrocrotonin (a nor-clerodane from Croton cajucara) on human lymphocytes

Trans-Dehydrocrotonin, a 19-nor-clerodane, is the major norditerpene obtained from Croton cajucara, a Brazilian medicinal plant which presents important biological effects, such as antineoplastic and antiulcerogenic activities. In this work, we analyzed the effect of this sesquiterpene lactone on no...

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Detalles Bibliográficos
Autores principales:	Lemos, Telma M. A. M., Aoyama, Hiroshi, Cavagis, Alexandre D. M.
Formato:	Articulo Comunicacion
Lenguaje:	Inglés
Publicado:	2008
Materias:	Farmacia cytotoxicity; human lymphocytes; phosphatase; trans-dehydrocrotonin Farmacología Plantas medicinales Test de Citotoxicidad
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/7712 http://www.latamjpharm.org/trabajos/27/6/LAJOP_27_6_2_5_R7GLR4U085.pdf
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-7712
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institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Farmacia cytotoxicity; human lymphocytes; phosphatase; trans-dehydrocrotonin Farmacología Plantas medicinales Test de Citotoxicidad
spellingShingle	Farmacia cytotoxicity; human lymphocytes; phosphatase; trans-dehydrocrotonin Farmacología Plantas medicinales Test de Citotoxicidad Lemos, Telma M. A. M. Aoyama, Hiroshi Cavagis, Alexandre D. M. Cytotoxicity of dehydrocrotonin (a nor-clerodane from Croton cajucara) on human lymphocytes
topic_facet	Farmacia cytotoxicity; human lymphocytes; phosphatase; trans-dehydrocrotonin Farmacología Plantas medicinales Test de Citotoxicidad
description	Trans-Dehydrocrotonin, a 19-nor-clerodane, is the major norditerpene obtained from Croton cajucara, a Brazilian medicinal plant which presents important biological effects, such as antineoplastic and antiulcerogenic activities. In this work, we analyzed the effect of this sesquiterpene lactone on normal human lymphocytes. The cell viability was verified after treatment for 24 and 72 h with trans-dehydrocrotonin, in the presence and absence of phytohemagglutin (specific mitogen for this cell), through three endpoints to assess cytotoxicity in vitro: MTT reduction (mitochondrial function), protein quantification (cell number) and phosphatase activity (cell metabolism). When the cells were treated with dehydrocrotonin in the presence of mitogen, no toxic effect was observed. Nevertheless, in the absence of mitogen, the IC50 was 450 μM for MTT reduction and phosphatase activity. Moreover, in this condition, trans-dehydrocrotonin caused stimulation of protein content from 100 μM. Our results suggest that phytohemagglutin protects human lymphocytes against the trans-dehydrocrotonin toxic effect.
format	Articulo Comunicacion
author	Lemos, Telma M. A. M. Aoyama, Hiroshi Cavagis, Alexandre D. M.
author_facet	Lemos, Telma M. A. M. Aoyama, Hiroshi Cavagis, Alexandre D. M.
author_sort	Lemos, Telma M. A. M.
title	Cytotoxicity of dehydrocrotonin (a nor-clerodane from Croton cajucara) on human lymphocytes
title_short	Cytotoxicity of dehydrocrotonin (a nor-clerodane from Croton cajucara) on human lymphocytes
title_full	Cytotoxicity of dehydrocrotonin (a nor-clerodane from Croton cajucara) on human lymphocytes
title_fullStr	Cytotoxicity of dehydrocrotonin (a nor-clerodane from Croton cajucara) on human lymphocytes
title_full_unstemmed	Cytotoxicity of dehydrocrotonin (a nor-clerodane from Croton cajucara) on human lymphocytes
title_sort	cytotoxicity of dehydrocrotonin (a nor-clerodane from croton cajucara) on human lymphocytes
publishDate	2008
url	http://sedici.unlp.edu.ar/handle/10915/7712 http://www.latamjpharm.org/trabajos/27/6/LAJOP_27_6_2_5_R7GLR4U085.pdf
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Cytotoxicity of dehydrocrotonin (a nor-clerodane from Croton cajucara) on human lymphocytes

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