Analysis of the influence of five variables on an established immunoaffinity chromatography procedure to purify a picchia pastoris yeast derived-HBsAg for pharmaceutical use
Immunoaffinity chromatography (IAC) has a wide application in protein purification. The aim of the study was to investigate the influence of five operational factors on an IAC procedure to purify a yeast derived-Hepatitis B surface antigen (rHBsAg) for pharmaceutical use. The immunosorbent adsorptio...
Guardado en:
| Autores principales: | , , , , , , , , , , , , , |
|---|---|
| Formato: | Articulo |
| Lenguaje: | Inglés |
| Publicado: |
2008
|
| Materias: | |
| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/7705 http://www.latamjpharm.org/trabajos/27/6/LAJOP_27_6_1_12_LD68X28956.pdf |
| Aporte de: |
| id |
I19-R120-10915-7705 |
|---|---|
| record_format |
dspace |
| institution |
Universidad Nacional de La Plata |
| institution_str |
I-19 |
| repository_str |
R-120 |
| collection |
SEDICI (UNLP) |
| language |
Inglés |
| topic |
Farmacia hepatitis B surface antigen; immunoaffinity chromatography; purification of rHBsAg; protein purification Inmunología Farmacología Bioquímica Cromatografía |
| spellingShingle |
Farmacia hepatitis B surface antigen; immunoaffinity chromatography; purification of rHBsAg; protein purification Inmunología Farmacología Bioquímica Cromatografía Valdes, Rodolfo Medina, Yenisley Fernández, Eutimio G. Geada, Déborah Ferro, Williams Álvarez, Tatiana Padilla, Sigifredo Guevara, Yaryse Tamayo, Andrés Gomez, Leonardo Montero, José Figueroa, Alejandro Calas, Gisela García A., Cristina Analysis of the influence of five variables on an established immunoaffinity chromatography procedure to purify a picchia pastoris yeast derived-HBsAg for pharmaceutical use |
| topic_facet |
Farmacia hepatitis B surface antigen; immunoaffinity chromatography; purification of rHBsAg; protein purification Inmunología Farmacología Bioquímica Cromatografía |
| description |
Immunoaffinity chromatography (IAC) has a wide application in protein purification. The aim of the study was to investigate the influence of five operational factors on an IAC procedure to purify a yeast derived-Hepatitis B surface antigen (rHBsAg) for pharmaceutical use. The immunosorbent adsorption
capacity of the rHBsAg is affected at 4 °C. The applied antigen concentration, 100-1000 μg mL<sup>-1</sup>,
does not have influence on this IAC efficiency. The residence time, applied antigen amount and column geometry have a significant influence on the adsorption and elution capacity, and recovery of the rHBsAg. There is a marked retention of the rHBsAg into the matrix, which is reduced at residence times higher than 2 h. A high height/diameter ratio of the column, 3.75, reduces the antigen adsorption to the matrix but increases the recovery and the productivity for this high molecular weight (multiple chemical forms) antigen and a high affinity constant ligand. The solution of the rHBsAg retention into the matrix could increase by 30% the active pharmaceutical ingredient purification and the Hepatitis B vaccine production. |
| format |
Articulo Articulo |
| author |
Valdes, Rodolfo Medina, Yenisley Fernández, Eutimio G. Geada, Déborah Ferro, Williams Álvarez, Tatiana Padilla, Sigifredo Guevara, Yaryse Tamayo, Andrés Gomez, Leonardo Montero, José Figueroa, Alejandro Calas, Gisela García A., Cristina |
| author_facet |
Valdes, Rodolfo Medina, Yenisley Fernández, Eutimio G. Geada, Déborah Ferro, Williams Álvarez, Tatiana Padilla, Sigifredo Guevara, Yaryse Tamayo, Andrés Gomez, Leonardo Montero, José Figueroa, Alejandro Calas, Gisela García A., Cristina |
| author_sort |
Valdes, Rodolfo |
| title |
Analysis of the influence of five variables on an established immunoaffinity chromatography procedure to purify a picchia pastoris yeast derived-HBsAg for pharmaceutical use |
| title_short |
Analysis of the influence of five variables on an established immunoaffinity chromatography procedure to purify a picchia pastoris yeast derived-HBsAg for pharmaceutical use |
| title_full |
Analysis of the influence of five variables on an established immunoaffinity chromatography procedure to purify a picchia pastoris yeast derived-HBsAg for pharmaceutical use |
| title_fullStr |
Analysis of the influence of five variables on an established immunoaffinity chromatography procedure to purify a picchia pastoris yeast derived-HBsAg for pharmaceutical use |
| title_full_unstemmed |
Analysis of the influence of five variables on an established immunoaffinity chromatography procedure to purify a picchia pastoris yeast derived-HBsAg for pharmaceutical use |
| title_sort |
analysis of the influence of five variables on an established immunoaffinity chromatography procedure to purify a picchia pastoris yeast derived-hbsag for pharmaceutical use |
| publishDate |
2008 |
| url |
http://sedici.unlp.edu.ar/handle/10915/7705 http://www.latamjpharm.org/trabajos/27/6/LAJOP_27_6_1_12_LD68X28956.pdf |
| work_keys_str_mv |
AT valdesrodolfo analysisoftheinfluenceoffivevariablesonanestablishedimmunoaffinitychromatographyproceduretopurifyapicchiapastorisyeastderivedhbsagforpharmaceuticaluse AT medinayenisley analysisoftheinfluenceoffivevariablesonanestablishedimmunoaffinitychromatographyproceduretopurifyapicchiapastorisyeastderivedhbsagforpharmaceuticaluse AT fernandezeutimiog analysisoftheinfluenceoffivevariablesonanestablishedimmunoaffinitychromatographyproceduretopurifyapicchiapastorisyeastderivedhbsagforpharmaceuticaluse AT geadadeborah analysisoftheinfluenceoffivevariablesonanestablishedimmunoaffinitychromatographyproceduretopurifyapicchiapastorisyeastderivedhbsagforpharmaceuticaluse AT ferrowilliams analysisoftheinfluenceoffivevariablesonanestablishedimmunoaffinitychromatographyproceduretopurifyapicchiapastorisyeastderivedhbsagforpharmaceuticaluse AT alvareztatiana analysisoftheinfluenceoffivevariablesonanestablishedimmunoaffinitychromatographyproceduretopurifyapicchiapastorisyeastderivedhbsagforpharmaceuticaluse AT padillasigifredo analysisoftheinfluenceoffivevariablesonanestablishedimmunoaffinitychromatographyproceduretopurifyapicchiapastorisyeastderivedhbsagforpharmaceuticaluse AT guevarayaryse analysisoftheinfluenceoffivevariablesonanestablishedimmunoaffinitychromatographyproceduretopurifyapicchiapastorisyeastderivedhbsagforpharmaceuticaluse AT tamayoandres analysisoftheinfluenceoffivevariablesonanestablishedimmunoaffinitychromatographyproceduretopurifyapicchiapastorisyeastderivedhbsagforpharmaceuticaluse AT gomezleonardo analysisoftheinfluenceoffivevariablesonanestablishedimmunoaffinitychromatographyproceduretopurifyapicchiapastorisyeastderivedhbsagforpharmaceuticaluse AT monterojose analysisoftheinfluenceoffivevariablesonanestablishedimmunoaffinitychromatographyproceduretopurifyapicchiapastorisyeastderivedhbsagforpharmaceuticaluse AT figueroaalejandro analysisoftheinfluenceoffivevariablesonanestablishedimmunoaffinitychromatographyproceduretopurifyapicchiapastorisyeastderivedhbsagforpharmaceuticaluse AT calasgisela analysisoftheinfluenceoffivevariablesonanestablishedimmunoaffinitychromatographyproceduretopurifyapicchiapastorisyeastderivedhbsagforpharmaceuticaluse AT garciaacristina analysisoftheinfluenceoffivevariablesonanestablishedimmunoaffinitychromatographyproceduretopurifyapicchiapastorisyeastderivedhbsagforpharmaceuticaluse |
| bdutipo_str |
Repositorios |
| _version_ |
1764820484994629634 |