Correlation between Diazepam in plasma and dose in patients in long-term treatment
Diazepam, a benzodiazepine drug, has many therapeutic uses but little is known about the diazepam dose/plasma level ratio when the drug is administered for a long period of time. In the present study we determined plasma diazepam concentration in 26 patients receiving 5 and 10 mg/day of the drug. A...
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| Autores principales: | , , , |
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| Formato: | Articulo Comunicacion |
| Lenguaje: | Inglés |
| Publicado: |
2008
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| Materias: | |
| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/7689 http://www.latamjpharm.org/resumenes/27/5/LAJOP_27_5_2_4.pdf |
| Aporte de: |
| Sumario: | Diazepam, a benzodiazepine drug, has many therapeutic uses but little is known about the diazepam dose/plasma level ratio when the drug is administered for a long period of time. In the present study we determined plasma diazepam concentration in 26 patients receiving 5 and 10 mg/day of the drug.
A gas chromatography/electron-capture detector method was validated for diazepam quantification in plasma using an OV 17-3% in Chromosorb W 80/100 mesh column. The use of 70:30 n-hexane: dichoromethane (v/v) as extraction solvent yielded good results. The following data were obtained: linearity from 10 to 1000 ng mL–1, detection and quantification limits of 5 and 10 ng.mL–1, respectively; intra and interassay average precision of 4.6 and 7.9%, respectively, mean recovery of 80.6%. The drug remained stable in the plasma sample for at least 30 days when stored at -20 °C. The relation between dose and plasma concentration did not increase in linearity when the dose was increased. |
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