Osteoblast Behavior on Novel Porous Polymeric Scaffolds

Current efforts in bone tissue engineering have as one focus the search for a scaffold material that supports osteoblast proliferation, matrix mineralization, and, ultimately, bone formation. Electrospraying of polymer solutions has enabled the engineering of porous materials to meet current challen...

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Detalles Bibliográficos
Autores principales: Fernández, Juan Manuel, Cortizo, María Susana, Cortizo, Ana María, Abraham, Gustavo A.
Formato: Articulo
Lenguaje:Inglés
Publicado: 2011
Materias:
Ciencias Exactas
Ingeniería de Tejidos
Electrospraying, Bone Tissue engineering, Adhesion, Proliferation, Alkaline Phosphatase
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/76784
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-76784
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
Ingeniería de Tejidos
Electrospraying, Bone Tissue engineering, Adhesion, Proliferation, Alkaline Phosphatase
spellingShingle Ciencias Exactas
Ingeniería de Tejidos
Electrospraying, Bone Tissue engineering, Adhesion, Proliferation, Alkaline Phosphatase
Fernández, Juan Manuel
Cortizo, María Susana
Cortizo, Ana María
Abraham, Gustavo A.
Osteoblast Behavior on Novel Porous Polymeric Scaffolds
topic_facet Ciencias Exactas
Ingeniería de Tejidos
Electrospraying, Bone Tissue engineering, Adhesion, Proliferation, Alkaline Phosphatase
description Current efforts in bone tissue engineering have as one focus the search for a scaffold material that supports osteoblast proliferation, matrix mineralization, and, ultimately, bone formation. Electrospraying of polymer solutions has enabled the engineering of porous materials to meet current challenges in bone replacement therapies. Porous scaffolds of poly( -caprolactone)/poly(diisopropyl fumarate) compatibilized blend for bone tissue engineering were obtained by electrospraying technique in order to create a better osteophilic environment for the growth and differentiation of osteoblasts. Non-porous films having smooth surface were obtained by casting and used for comparison purposes. Studies on cell-scaffold interaction were carried out by culturing two osteoblastlike cell lines, MC3T3E1 and UMR106, on three-dimensional scaffolds and two-dimensional films. Growth, proliferation, and differentiation (alkaline phosphatase activity) of osteoblasts, were assessed. Scaffolds displayed a highly porous structure with interconnected pores formed by polymer microparticles, and higher hydrophobicity than the observed in non-porous films. The adhesion, proliferation and alkaline phosphatase activity of cells grown on the porous scaffolds increased significantly in comparison to those observed on flat films. The rough surface morphology of this novel scaffold enhances osteoblast response. These results suggest that electrosprayed porous scaffolds may be potentially used as tissue engineering scaffolds with high bone regenerative efficacy.
format Articulo
Articulo
author Fernández, Juan Manuel
Cortizo, María Susana
Cortizo, Ana María
Abraham, Gustavo A.
author_facet Fernández, Juan Manuel
Cortizo, María Susana
Cortizo, Ana María
Abraham, Gustavo A.
author_sort Fernández, Juan Manuel
title Osteoblast Behavior on Novel Porous Polymeric Scaffolds
title_short Osteoblast Behavior on Novel Porous Polymeric Scaffolds
title_full Osteoblast Behavior on Novel Porous Polymeric Scaffolds
title_fullStr Osteoblast Behavior on Novel Porous Polymeric Scaffolds
title_full_unstemmed Osteoblast Behavior on Novel Porous Polymeric Scaffolds
title_sort osteoblast behavior on novel porous polymeric scaffolds
publishDate 2011
url http://sedici.unlp.edu.ar/handle/10915/76784
work_keys_str_mv AT fernandezjuanmanuel osteoblastbehavioronnovelporouspolymericscaffolds
AT cortizomariasusana osteoblastbehavioronnovelporouspolymericscaffolds
AT cortizoanamaria osteoblastbehavioronnovelporouspolymericscaffolds
AT abrahamgustavoa osteoblastbehavioronnovelporouspolymericscaffolds
bdutipo_str Repositorios
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