Insulin-mimetic action of vanadium compounds on osteoblast-like cells in culture

Vanadium compounds mimic insulin actions in different cell types. The present study concerns the insulin-like effects of three vanadium(V) derivatives and one vanadium(IV) complex on osteoblast-like (UMR106 and MC3T3E1) cells in culture. The vanadium oxalate and vanadium citrate complexes hydrolyzed...

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Autores principales: Etcheverry, Susana B., Crans, D. C., Keramidas, A. D., Cortizo, Ana María
Formato: Articulo
Lenguaje:Inglés
Publicado: 1997
Materias:
Ciencias Exactas
vanadate, aqueous vanadium chemistry, vanastability, spectroscopy, proliferation, differentiation, glucose consumption, protein content, alkaline phosphatase, osteoblast-like cells
Química
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/76585
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-76585
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
vanadate, aqueous vanadium chemistry, vanastability, spectroscopy, proliferation, differentiation, glucose consumption, protein content, alkaline phosphatase, osteoblast-like cells
Química
spellingShingle Ciencias Exactas
vanadate, aqueous vanadium chemistry, vanastability, spectroscopy, proliferation, differentiation, glucose consumption, protein content, alkaline phosphatase, osteoblast-like cells
Química
Etcheverry, Susana B.
Crans, D. C.
Keramidas, A. D.
Cortizo, Ana María
Insulin-mimetic action of vanadium compounds on osteoblast-like cells in culture
topic_facet Ciencias Exactas
vanadate, aqueous vanadium chemistry, vanastability, spectroscopy, proliferation, differentiation, glucose consumption, protein content, alkaline phosphatase, osteoblast-like cells
Química
description Vanadium compounds mimic insulin actions in different cell types. The present study concerns the insulin-like effects of three vanadium(V) derivatives and one vanadium(IV) complex on osteoblast-like (UMR106 and MC3T3E1) cells in culture. The vanadium oxalate and vanadium citrate complexes hydrolyzed completely under the culture conditions, whereas more than 40% of the vanadium tartrate and nitrilotriacetate complexes remained. Vanadate, as well as vanadium oxalate, citrate, and tartrate complexes enhanced cell proliferation (as measured by the crystal violet assay), glucose consumption, and protein content in UMR106 and MC3T3E1 osteoblast-like cells. The vanadium nitrilotriacetate complex (the only peroxo complex tested) stimulated cell proliferation in UMR106 but not in MC3T3E1 cells. This derivative strongly transformed the morphology of the MC3T3E1 cells. All vanadium(V) compounds inhibited cell differentiation (alkaline phosphatase activity) in UMR106 cells. Our data are consistent with the interpretation that vanadium oxalate and citrate complexes hydrolyze to vanadate. Vanadium nitrilotriacetate would appear to be toxic for normal MC3T3E1 osteoblasts. In contrast, the vanadium tartrate complex induced a proliferative effect; however, it did not alter cell differentiation.
format Articulo
Articulo
author Etcheverry, Susana B.
Crans, D. C.
Keramidas, A. D.
Cortizo, Ana María
author_facet Etcheverry, Susana B.
Crans, D. C.
Keramidas, A. D.
Cortizo, Ana María
author_sort Etcheverry, Susana B.
title Insulin-mimetic action of vanadium compounds on osteoblast-like cells in culture
title_short Insulin-mimetic action of vanadium compounds on osteoblast-like cells in culture
title_full Insulin-mimetic action of vanadium compounds on osteoblast-like cells in culture
title_fullStr Insulin-mimetic action of vanadium compounds on osteoblast-like cells in culture
title_full_unstemmed Insulin-mimetic action of vanadium compounds on osteoblast-like cells in culture
title_sort insulin-mimetic action of vanadium compounds on osteoblast-like cells in culture
publishDate 1997
url http://sedici.unlp.edu.ar/handle/10915/76585
work_keys_str_mv AT etcheverrysusanab insulinmimeticactionofvanadiumcompoundsonosteoblastlikecellsinculture
AT cransdc insulinmimeticactionofvanadiumcompoundsonosteoblastlikecellsinculture
AT keramidasad insulinmimeticactionofvanadiumcompoundsonosteoblastlikecellsinculture
AT cortizoanamaria insulinmimeticactionofvanadiumcompoundsonosteoblastlikecellsinculture
bdutipo_str Repositorios
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