Preliminary Characterization of Thin Biofilms by Optical Microscopy

A simple non-invasive technique has been used that employs conventional optical microscopy and a glass flow cell to observe biofilms formed on opaque thin substrata. The technique allows the roughness of the biofilm and the substratum to be evaluated, and the biofilm thickness to be easily measured....

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Autores principales: Cortizo, María Cecilia, Fernández Lorenzo de Mele, Mónica Alicia
Formato: Articulo
Lenguaje:Inglés
Publicado: 2000
Materias:
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/76479
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id I19-R120-10915-76479
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Odontología
microscopy; biofilm; bacteria; colonization; bacteria! attachment; Pseudomonas fluorescens
Materiales Biomédicos y Dentales
Biofilmes
spellingShingle Odontología
microscopy; biofilm; bacteria; colonization; bacteria! attachment; Pseudomonas fluorescens
Materiales Biomédicos y Dentales
Biofilmes
Cortizo, María Cecilia
Fernández Lorenzo de Mele, Mónica Alicia
Preliminary Characterization of Thin Biofilms by Optical Microscopy
topic_facet Odontología
microscopy; biofilm; bacteria; colonization; bacteria! attachment; Pseudomonas fluorescens
Materiales Biomédicos y Dentales
Biofilmes
description A simple non-invasive technique has been used that employs conventional optical microscopy and a glass flow cell to observe biofilms formed on opaque thin substrata. The technique allows the roughness of the biofilm and the substratum to be evaluated, and the biofilm thickness to be easily measured. The biofilm density may be quantified through colour gradients. In addition, sorne details of biofilm growth processes like the formation of water channels and pores, and interactions between planktonic and sessile cells can be visualized. Results related to the development of thin biofilms and their response to the environment under different conditions are reported. Pure and mixed microbial cultures and different solid substrata were assessed.
format Articulo
Articulo
author Cortizo, María Cecilia
Fernández Lorenzo de Mele, Mónica Alicia
author_facet Cortizo, María Cecilia
Fernández Lorenzo de Mele, Mónica Alicia
author_sort Cortizo, María Cecilia
title Preliminary Characterization of Thin Biofilms by Optical Microscopy
title_short Preliminary Characterization of Thin Biofilms by Optical Microscopy
title_full Preliminary Characterization of Thin Biofilms by Optical Microscopy
title_fullStr Preliminary Characterization of Thin Biofilms by Optical Microscopy
title_full_unstemmed Preliminary Characterization of Thin Biofilms by Optical Microscopy
title_sort preliminary characterization of thin biofilms by optical microscopy
publishDate 2000
url http://sedici.unlp.edu.ar/handle/10915/76479
work_keys_str_mv AT cortizomariacecilia preliminarycharacterizationofthinbiofilmsbyopticalmicroscopy
AT fernandezlorenzodemelemonicaalicia preliminarycharacterizationofthinbiofilmsbyopticalmicroscopy
bdutipo_str Repositorios
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