Microstructural characteristics of thin biofilms through optical and scanning electron microscopy

The combination of a conventional optical microscope with a specially designed glass flow cell was used to visualize ‘in situ’ biofilms formed on opaque thin biomaterials through a simple non-invasive way (optical microscopy of thin biofilms, OMTB). Comparisons of OMTB with scanning electron microsc...

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Detalles Bibliográficos
Autores principales: Cortizo, María Cecilia, Fernández Lorenzo de Mele, Mónica Alicia
Formato: Articulo
Lenguaje:Inglés
Publicado: 2003
Materias:
Ciencias Exactas
Materiales Biomédicos y Dentales
Biofilmes
biofilm thickness, dental materials, optical microscopy, oral microorganisms, roughness, scanning electron microscopy
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/76472
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-76472
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
Materiales Biomédicos y Dentales
Biofilmes
biofilm thickness, dental materials, optical microscopy, oral microorganisms, roughness, scanning electron microscopy
spellingShingle Ciencias Exactas
Materiales Biomédicos y Dentales
Biofilmes
biofilm thickness, dental materials, optical microscopy, oral microorganisms, roughness, scanning electron microscopy
Cortizo, María Cecilia
Fernández Lorenzo de Mele, Mónica Alicia
Microstructural characteristics of thin biofilms through optical and scanning electron microscopy
topic_facet Ciencias Exactas
Materiales Biomédicos y Dentales
Biofilmes
biofilm thickness, dental materials, optical microscopy, oral microorganisms, roughness, scanning electron microscopy
description The combination of a conventional optical microscope with a specially designed glass flow cell was used to visualize ‘in situ’ biofilms formed on opaque thin biomaterials through a simple non-invasive way (optical microscopy of thin biofilms, OMTB). Comparisons of OMTB with scanning electron microscopy (SEM) images were made. Thin metallic dental biomaterials were used as substrata. They were immersed in a synthetic saliva and in a modified Mitis–Salivarius medium inoculated with a consortium of oral microorganisms. To study the effect of bacterial motility, Pseudomonas fluorescens cultures were also used. The processes which give rise to the formation of the biofilm were monitored through OMTB. Biofilm microstructures like pores, water channels, streamers and chains of Streptococci, attached to the surface or floating in the viscous interfacial environment, could be distinguished. Thickness and roughness of the biofilms formed on thin substrata could also be evaluated. Distortions introduced by pretreatments carried out to prepare biological materials for SEM observations could be detected by comparing OMTB and SEM images. SEM images (obtained at high magnification but ex situ, not in real time and with pretreatment of the samples) and OMTB images (obtained in situ, without pretreatments, in real time but at low magnification) in combination provided complementary information to study biofilm processes on thin substrata.
format Articulo
Articulo
author Cortizo, María Cecilia
Fernández Lorenzo de Mele, Mónica Alicia
author_facet Cortizo, María Cecilia
Fernández Lorenzo de Mele, Mónica Alicia
author_sort Cortizo, María Cecilia
title Microstructural characteristics of thin biofilms through optical and scanning electron microscopy
title_short Microstructural characteristics of thin biofilms through optical and scanning electron microscopy
title_full Microstructural characteristics of thin biofilms through optical and scanning electron microscopy
title_fullStr Microstructural characteristics of thin biofilms through optical and scanning electron microscopy
title_full_unstemmed Microstructural characteristics of thin biofilms through optical and scanning electron microscopy
title_sort microstructural characteristics of thin biofilms through optical and scanning electron microscopy
publishDate 2003
url http://sedici.unlp.edu.ar/handle/10915/76472
work_keys_str_mv AT cortizomariacecilia microstructuralcharacteristicsofthinbiofilmsthroughopticalandscanningelectronmicroscopy
AT fernandezlorenzodemelemonicaalicia microstructuralcharacteristicsofthinbiofilmsthroughopticalandscanningelectronmicroscopy
bdutipo_str Repositorios
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