Vanadate-induced nitric oxide production: role in osteoblast growth and differentiation

Nitric oxide NO. has been shown to act as a mediator of cytokines in bone tissue. We have previously demonstrated that vanadium compounds are insulin- and growth factor-mimetic compounds in osteoblasts in culture, although high doses are toxic to these cells. In this study, we measured NO productio...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Cortizo, Ana María, Caporossi, Mariana, Lettieri, Gabriela, Etcheverry, Susana B.
Formato: Articulo
Lenguaje:Inglés
Publicado: 2000
Materias:
Ciencias Exactas
Nitric oxide ŽNO.; Nitric oxide ŽNO. synthases; Vanadium; Growth; Osteoblast differentiation; Bone; Cytotoxicity
Química
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/76265
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-76265
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
Nitric oxide ŽNO.; Nitric oxide ŽNO. synthases; Vanadium; Growth; Osteoblast differentiation; Bone; Cytotoxicity
Química
spellingShingle Ciencias Exactas
Nitric oxide ŽNO.; Nitric oxide ŽNO. synthases; Vanadium; Growth; Osteoblast differentiation; Bone; Cytotoxicity
Química
Cortizo, Ana María
Caporossi, Mariana
Lettieri, Gabriela
Etcheverry, Susana B.
Vanadate-induced nitric oxide production: role in osteoblast growth and differentiation
topic_facet Ciencias Exactas
Nitric oxide ŽNO.; Nitric oxide ŽNO. synthases; Vanadium; Growth; Osteoblast differentiation; Bone; Cytotoxicity
Química
description Nitric oxide NO. has been shown to act as a mediator of cytokines in bone tissue. We have previously demonstrated that vanadium compounds are insulin- and growth factor-mimetic compounds in osteoblasts in culture, although high doses are toxic to these cells. In this study, we measured NO production in two osteoblast-like cells UMR106 and MC3T3E1. incubated with different concentrations 2.5–100 mM. of vanadate. Vanadate induced NO release in a biphasic manner, with levels being significantly increased at concentrations over 50 mM. The NO donor, sodium nitroprusside, mimicked the vanadate effect: it inhibited cell growth and alkaline phosphatase activity in a dose-dependent manner. Vanadate enhanced the NO synthases, the endothelial and inducible eNOS and iNOS. isoforms, in a dose-dependent manner. Experiments performed with the ionophore A23187 and EGTA suggested that vanadate-induced NO production involves Ca2q-dependent and -independent mechanisms. Altogether, our results suggest that NO may play a critical role in the bioactivity of vanadium in osteoblast-like cells.
format Articulo
Articulo
author Cortizo, Ana María
Caporossi, Mariana
Lettieri, Gabriela
Etcheverry, Susana B.
author_facet Cortizo, Ana María
Caporossi, Mariana
Lettieri, Gabriela
Etcheverry, Susana B.
author_sort Cortizo, Ana María
title Vanadate-induced nitric oxide production: role in osteoblast growth and differentiation
title_short Vanadate-induced nitric oxide production: role in osteoblast growth and differentiation
title_full Vanadate-induced nitric oxide production: role in osteoblast growth and differentiation
title_fullStr Vanadate-induced nitric oxide production: role in osteoblast growth and differentiation
title_full_unstemmed Vanadate-induced nitric oxide production: role in osteoblast growth and differentiation
title_sort vanadate-induced nitric oxide production: role in osteoblast growth and differentiation
publishDate 2000
url http://sedici.unlp.edu.ar/handle/10915/76265
work_keys_str_mv AT cortizoanamaria vanadateinducednitricoxideproductionroleinosteoblastgrowthanddifferentiation
AT caporossimariana vanadateinducednitricoxideproductionroleinosteoblastgrowthanddifferentiation
AT lettierigabriela vanadateinducednitricoxideproductionroleinosteoblastgrowthanddifferentiation
AT etcheverrysusanab vanadateinducednitricoxideproductionroleinosteoblastgrowthanddifferentiation
bdutipo_str Repositorios
_version_ 1764820487989362689

Ejemplares similares