Proliferative and morphological changes induced by vanadium compounds on Swiss 3T3 fibroblasts

Vanadium compounds are shown to have a mitogenic effect on fibroblast cells. The effects of vanadate, vanadyl and pervanadate on the proliferation and morphological changes of Swiss 3T3 cells in culture are compared. Vanadium derivatives induced cell proliferation in a biphasic manner, with a toxic-...

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Autores principales: Cortizo, Ana María, Sálice, Viviana C., Vescina, Cecilia M., Etcheverry, Susana B.
Formato: Articulo
Lenguaje:Inglés
Publicado: 1997
Materias:
Ciencias Exactas
cytotoxicity, fibroblasts, growth factors, proliferation, vanadium
Química
Vanadio
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/76264
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-76264
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
cytotoxicity, fibroblasts, growth factors, proliferation, vanadium
Química
Vanadio
spellingShingle Ciencias Exactas
cytotoxicity, fibroblasts, growth factors, proliferation, vanadium
Química
Vanadio
Cortizo, Ana María
Sálice, Viviana C.
Vescina, Cecilia M.
Etcheverry, Susana B.
Proliferative and morphological changes induced by vanadium compounds on Swiss 3T3 fibroblasts
topic_facet Ciencias Exactas
cytotoxicity, fibroblasts, growth factors, proliferation, vanadium
Química
Vanadio
description Vanadium compounds are shown to have a mitogenic effect on fibroblast cells. The effects of vanadate, vanadyl and pervanadate on the proliferation and morphological changes of Swiss 3T3 cells in culture are compared. Vanadium derivatives induced cell proliferation in a biphasic manner, with a toxic-like effect at doses over 50 mM, after 24 h of incubation. Vanadyl and vanadate were equally potent at 2.5–10 mM. At 50 mM vanadate inhibited cell proliferation, whereas slight inhibition was observed at 100 mM of vanadyl. At 10 mM pervanadate was as potent as vanadate and vanadyl in stimulating fibroblast proliferation, but no effect was observed at lower concentrations. A pronounced cytotoxic-like effect was induced by pervanadate at 50 mM. All of these effects were accompanied by morphological changes: transformation of fibroblast shape from polygonal to fusiform; retraction with cytoplasm condensation; and loss of lamellar processes. The magnitude of these transformations correlates with the potency of vanadium derivatives to induce a cytotoxic-like effect: pervanadate > vanadate > vanadyl. These data suggest that the oxidation state and coordination geometry of vanadium determine the degree of the cytotoxicity.
format Articulo
Articulo
author Cortizo, Ana María
Sálice, Viviana C.
Vescina, Cecilia M.
Etcheverry, Susana B.
author_facet Cortizo, Ana María
Sálice, Viviana C.
Vescina, Cecilia M.
Etcheverry, Susana B.
author_sort Cortizo, Ana María
title Proliferative and morphological changes induced by vanadium compounds on Swiss 3T3 fibroblasts
title_short Proliferative and morphological changes induced by vanadium compounds on Swiss 3T3 fibroblasts
title_full Proliferative and morphological changes induced by vanadium compounds on Swiss 3T3 fibroblasts
title_fullStr Proliferative and morphological changes induced by vanadium compounds on Swiss 3T3 fibroblasts
title_full_unstemmed Proliferative and morphological changes induced by vanadium compounds on Swiss 3T3 fibroblasts
title_sort proliferative and morphological changes induced by vanadium compounds on swiss 3t3 fibroblasts
publishDate 1997
url http://sedici.unlp.edu.ar/handle/10915/76264
work_keys_str_mv AT cortizoanamaria proliferativeandmorphologicalchangesinducedbyvanadiumcompoundsonswiss3t3fibroblasts
AT salicevivianac proliferativeandmorphologicalchangesinducedbyvanadiumcompoundsonswiss3t3fibroblasts
AT vescinaceciliam proliferativeandmorphologicalchangesinducedbyvanadiumcompoundsonswiss3t3fibroblasts
AT etcheverrysusanab proliferativeandmorphologicalchangesinducedbyvanadiumcompoundsonswiss3t3fibroblasts
bdutipo_str Repositorios
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