Synthesis of a new vanadyl(IV) complex with trehalose (TreVO): insulin-mimetic activities in osteoblast-like cells in culture

Vanadium compounds show interesting biological and pharmacological properties. Some of them display insulin-mimetic effects and others produce antitumor actions. The bioactivity of vanadium is present in inorganic species like the vanadyl(IV) cation or vanadate( V) anion. Nevertheless, the developme...

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Detalles Bibliográficos
Autores principales:	Barrio, Daniel Alejandro, Williams, Patricia Ana María, Cortizo, Ana María, Etcheverry, Susana B.
Formato:	Articulo
Lenguaje:	Español
Publicado:	2003
Materias:	Ciencias Exactas diabetes mellitus; insulin mimics; osteoblasts; signal transduction; vanadium Insulina Bioquímica
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/76081
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-76081
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Español
topic	Ciencias Exactas diabetes mellitus; insulin mimics; osteoblasts; signal transduction; vanadium Insulina Bioquímica
spellingShingle	Ciencias Exactas diabetes mellitus; insulin mimics; osteoblasts; signal transduction; vanadium Insulina Bioquímica Barrio, Daniel Alejandro Williams, Patricia Ana María Cortizo, Ana María Etcheverry, Susana B. Synthesis of a new vanadyl(IV) complex with trehalose (TreVO): insulin-mimetic activities in osteoblast-like cells in culture
topic_facet	Ciencias Exactas diabetes mellitus; insulin mimics; osteoblasts; signal transduction; vanadium Insulina Bioquímica
description	Vanadium compounds show interesting biological and pharmacological properties. Some of them display insulin-mimetic effects and others produce antitumor actions. The bioactivity of vanadium is present in inorganic species like the vanadyl(IV) cation or vanadate( V) anion. Nevertheless, the development of new vanadium derivatives with organic ligands which improve the beneficial actions and decrease the toxic effects is of great interest. On the other hand, the mechanisms involved in vanadium bioactivity are still poorly understood. A new vanadium complex of the vanadyl(IV) cation with the disaccharide trehalose (TreVO), Na6 [VO(Tre)2].4H2O, here reported, shows interesting insulin- mimetic properties in two osteoblast cell lines, a normal one (MC3T3E1) and a tumoral one (UMR106). The complex affected the proliferation of both cell lines in a different manner. On tumoral cells, TreVO caused a weak stimulation of growth at 5 lM but it inhibited cell proliferation in a dose-response manner between 50 and 100 lM. TreVO significantly inhibited UMR106 differentiation (15–25% of basal) in the range 5–100 lM. On normal osteoblasts, TreVO behaved as a mitogen at 5–25 lM. Different inhibitors of the MAPK pathway blocked this effect. At higher concentrations (75–100 lM), the complex was a weak inhibitor of the MC3T3E1 proliferation. Besides, TreVO enhanced glucose consumption by a mechanism independent of the PI3-kinase activation. In both cell lines, TreVO stimulated the ERK phosphorylation in a dose- and time-dependent manner. Different inhibitors (PD98059, wortmannin, vitamins C and E) partially decreased this effect, which was totally inhibited by their combination. These results suggest that TreVO could be a potential candidate for therapeutic treatments.
format	Articulo Articulo
author	Barrio, Daniel Alejandro Williams, Patricia Ana María Cortizo, Ana María Etcheverry, Susana B.
author_facet	Barrio, Daniel Alejandro Williams, Patricia Ana María Cortizo, Ana María Etcheverry, Susana B.
author_sort	Barrio, Daniel Alejandro
title	Synthesis of a new vanadyl(IV) complex with trehalose (TreVO): insulin-mimetic activities in osteoblast-like cells in culture
title_short	Synthesis of a new vanadyl(IV) complex with trehalose (TreVO): insulin-mimetic activities in osteoblast-like cells in culture
title_full	Synthesis of a new vanadyl(IV) complex with trehalose (TreVO): insulin-mimetic activities in osteoblast-like cells in culture
title_fullStr	Synthesis of a new vanadyl(IV) complex with trehalose (TreVO): insulin-mimetic activities in osteoblast-like cells in culture
title_full_unstemmed	Synthesis of a new vanadyl(IV) complex with trehalose (TreVO): insulin-mimetic activities in osteoblast-like cells in culture
title_sort	synthesis of a new vanadyl(iv) complex with trehalose (trevo): insulin-mimetic activities in osteoblast-like cells in culture
publishDate	2003
url	http://sedici.unlp.edu.ar/handle/10915/76081
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bdutipo_str	Repositorios
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Synthesis of a new vanadyl(IV) complex with trehalose (TreVO): insulin-mimetic activities in osteoblast-like cells in culture

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