Effect of Vanadium Compounds on the Lipid Organization of Liposomes and Cell Membranes

The influence of vanadate on the adsorption properties of Merocyanine 540 (MC540) to UMR cells was studied by means of specrofluorometry. An increment in the fluorescence was observed in the osteoblasts incubated with 0.1 mM vanadate. This effect could be interpreted in terms of vanadate inhibitory...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Bakás, Laura Susana, Verza, Georgina, Cortizo, Ana María
Formato: Articulo
Lenguaje:Inglés
Publicado: 2001
Materias:
Ciencias Médicas
Vanadio
vanadium compounds; liposomes; lipid membranes; membrane fluidity; Merocyanine 540; 1,6-diphenylhexatriene
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/76077
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-76077
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Médicas
Vanadio
vanadium compounds; liposomes; lipid membranes; membrane fluidity; Merocyanine 540; 1,6-diphenylhexatriene
spellingShingle Ciencias Médicas
Vanadio
vanadium compounds; liposomes; lipid membranes; membrane fluidity; Merocyanine 540; 1,6-diphenylhexatriene
Bakás, Laura Susana
Verza, Georgina
Cortizo, Ana María
Effect of Vanadium Compounds on the Lipid Organization of Liposomes and Cell Membranes
topic_facet Ciencias Médicas
Vanadio
vanadium compounds; liposomes; lipid membranes; membrane fluidity; Merocyanine 540; 1,6-diphenylhexatriene
description The influence of vanadate on the adsorption properties of Merocyanine 540 (MC540) to UMR cells was studied by means of specrofluorometry. An increment in the fluorescence was observed in the osteoblasts incubated with 0.1 mM vanadate. This effect could be interpreted in terms of vanadate inhibitory effects on aminotraslocase activity. However, vanadate promotes a similar behavior to that found in UMR 106 cells when it was added to lipid vesicles composed of phosphatidylcholine. The effect of vanadium in different oxidation states, such as vanadate(V) and vanadyl(IV) on lipid membrane properties was examined in large unilamellar vesicles by means of spectrofluorometry employing different probes. Merocyanine 540 and 1,6-diphenylhexatriene were used in order to sense the changes at interfacial and hydrophobic core of membranes, respectively. In contrast to vanadate, vanadyl decreased the fluorescence of MC540. Both vanadium compounds slightly perturbed the hydrocarbon core. The results can be interpreted by the specific adsorption of both compounds on the polar head groups of phospholipid and suggest a possible influence of vanadium compounds on the lipid organization of cell membranes.
format Articulo
Articulo
author Bakás, Laura Susana
Verza, Georgina
Cortizo, Ana María
author_facet Bakás, Laura Susana
Verza, Georgina
Cortizo, Ana María
author_sort Bakás, Laura Susana
title Effect of Vanadium Compounds on the Lipid Organization of Liposomes and Cell Membranes
title_short Effect of Vanadium Compounds on the Lipid Organization of Liposomes and Cell Membranes
title_full Effect of Vanadium Compounds on the Lipid Organization of Liposomes and Cell Membranes
title_fullStr Effect of Vanadium Compounds on the Lipid Organization of Liposomes and Cell Membranes
title_full_unstemmed Effect of Vanadium Compounds on the Lipid Organization of Liposomes and Cell Membranes
title_sort effect of vanadium compounds on the lipid organization of liposomes and cell membranes
publishDate 2001
url http://sedici.unlp.edu.ar/handle/10915/76077
work_keys_str_mv AT bakaslaurasusana effectofvanadiumcompoundsonthelipidorganizationofliposomesandcellmembranes
AT verzageorgina effectofvanadiumcompoundsonthelipidorganizationofliposomesandcellmembranes
AT cortizoanamaria effectofvanadiumcompoundsonthelipidorganizationofliposomesandcellmembranes
bdutipo_str Repositorios
_version_ 1764820487186153475

Ejemplares similares