Long-Term Fructose Intake Increases Adipogenic Potential: Evidence of Direct Effects of Fructose on Adipocyte Precursor Cells

We have previously addressed that fructose rich diet (FRD) intake for three weeks increases the adipogenic potential of stromal vascular fraction cells from the retroperitoneal adipose tissue (RPAT). We have now evaluated the effect of prolonged FRD intake (eight weeks) on metabolic parameters, numb...

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Detalles Bibliográficos
Autores principales: Zubiría, María Guillermina, Alzamendi, Ana, Moreno, Griselda, Rey, María Amanda, Spinedi, Eduardo Julio, Giovambattista, Andrés
Formato: Articulo
Lenguaje:Inglés
Publicado: 2016
Materias:
Ciencias Médicas
retroperitoneal adipose tissue; adipogenesis; SVF cells; precursor cell competency
Fructosa
Adipocitos
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/75627
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-75627
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Médicas
retroperitoneal adipose tissue; adipogenesis; SVF cells; precursor cell competency
Fructosa
Adipocitos
spellingShingle Ciencias Médicas
retroperitoneal adipose tissue; adipogenesis; SVF cells; precursor cell competency
Fructosa
Adipocitos
Zubiría, María Guillermina
Alzamendi, Ana
Moreno, Griselda
Rey, María Amanda
Spinedi, Eduardo Julio
Giovambattista, Andrés
Long-Term Fructose Intake Increases Adipogenic Potential: Evidence of Direct Effects of Fructose on Adipocyte Precursor Cells
topic_facet Ciencias Médicas
retroperitoneal adipose tissue; adipogenesis; SVF cells; precursor cell competency
Fructosa
Adipocitos
description We have previously addressed that fructose rich diet (FRD) intake for three weeks increases the adipogenic potential of stromal vascular fraction cells from the retroperitoneal adipose tissue (RPAT). We have now evaluated the effect of prolonged FRD intake (eight weeks) on metabolic parameters, number of adipocyte precursor cells (APCs) and in vitro adipogenic potential from control (CTR) and FRD adult male rats. Additionally, we have examined the direct fructose effects on the adipogenic capacity of normal APCs. FRD fed rats had increased plasma levels of insulin, triglyceride and leptin, and RPAT mass and adipocyte size. FACS studies showed higher APCs number and adipogenic potential in FRD RPAT pads; data is supported by high mRNA levels of competency markers: PPARγ2 and Zfp423. Complementary in vitro experiments indicate that fructose-exposed normal APCs displayed an overall increased adipogenic capacity. We conclude that the RPAT mass expansion observed in eight week-FRD fed rats depends on combined accelerated adipogenesis and adipocyte hypertrophy, partially due to a direct effect of fructose on APCs.
format Articulo
Articulo
author Zubiría, María Guillermina
Alzamendi, Ana
Moreno, Griselda
Rey, María Amanda
Spinedi, Eduardo Julio
Giovambattista, Andrés
author_facet Zubiría, María Guillermina
Alzamendi, Ana
Moreno, Griselda
Rey, María Amanda
Spinedi, Eduardo Julio
Giovambattista, Andrés
author_sort Zubiría, María Guillermina
title Long-Term Fructose Intake Increases Adipogenic Potential: Evidence of Direct Effects of Fructose on Adipocyte Precursor Cells
title_short Long-Term Fructose Intake Increases Adipogenic Potential: Evidence of Direct Effects of Fructose on Adipocyte Precursor Cells
title_full Long-Term Fructose Intake Increases Adipogenic Potential: Evidence of Direct Effects of Fructose on Adipocyte Precursor Cells
title_fullStr Long-Term Fructose Intake Increases Adipogenic Potential: Evidence of Direct Effects of Fructose on Adipocyte Precursor Cells
title_full_unstemmed Long-Term Fructose Intake Increases Adipogenic Potential: Evidence of Direct Effects of Fructose on Adipocyte Precursor Cells
title_sort long-term fructose intake increases adipogenic potential: evidence of direct effects of fructose on adipocyte precursor cells
publishDate 2016
url http://sedici.unlp.edu.ar/handle/10915/75627
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bdutipo_str Repositorios
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