Measurement of expansin activity and plant cell wall creep by using a commercial texture analyzer

Background: Expansins play an important role in cell wall metabolism and fruit softening. Determination of expansin activity is a challenging problem since it depends on measuring cell wall properties by using ad hoc extensometers, a fact that has strongly restricted its study. Then, the objective o...

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Autores principales: Perini, Mauro Alejandro, Sin, Ignacio, Martínez, Gustavo Adolfo, Civello, Pedro Marcos
Formato: Articulo
Lenguaje:Inglés
Publicado: 2017
Materias:
Ciencias Agrarias
acid growth, cell wall metabolism, cucumber, hypocotyls, extensometers, fruit softening, fruit softening, hypocotyls acid growth, plant cell wall properties, ripening, strawberry, tomato
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/74669
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-74669
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Agrarias
acid growth, cell wall metabolism, cucumber, hypocotyls, extensometers, fruit softening, fruit softening, hypocotyls acid growth, plant cell wall properties, ripening, strawberry, tomato
spellingShingle Ciencias Agrarias
acid growth, cell wall metabolism, cucumber, hypocotyls, extensometers, fruit softening, fruit softening, hypocotyls acid growth, plant cell wall properties, ripening, strawberry, tomato
Perini, Mauro Alejandro
Sin, Ignacio
Martínez, Gustavo Adolfo
Civello, Pedro Marcos
Measurement of expansin activity and plant cell wall creep by using a commercial texture analyzer
topic_facet Ciencias Agrarias
acid growth, cell wall metabolism, cucumber, hypocotyls, extensometers, fruit softening, fruit softening, hypocotyls acid growth, plant cell wall properties, ripening, strawberry, tomato
description Background: Expansins play an important role in cell wall metabolism and fruit softening. Determination of expansin activity is a challenging problem since it depends on measuring cell wall properties by using ad hoc extensometers, a fact that has strongly restricted its study. Then, the objective of the work was to adapt a methodology to measure cell wall creep and expansin activity using a commercial texture meter, equipped with miniature tensile grips and an ad hoc cuvette of easy construction. Results: It was possible to measure hypocotyls acid growth and expansin activity in a reliable and reproducible way, using a commercial texture meter, common equipment found in laboratories of food science or postharvest technology. Expansin activity was detected in protein extracts from cucumber hypocotyls, tomato and strawberry fruits, and statistical differences in expansin activity were found in both fruit models at different ripening stages. Conclusions: The possibility of measuring expansin activity following this adapted protocol with a commercial texture meter could contribute to ease and increase the analysis of expansin in different systems, leading to a better understanding of the properties of these proteins under different experimental conditions.
format Articulo
Articulo
author Perini, Mauro Alejandro
Sin, Ignacio
Martínez, Gustavo Adolfo
Civello, Pedro Marcos
author_facet Perini, Mauro Alejandro
Sin, Ignacio
Martínez, Gustavo Adolfo
Civello, Pedro Marcos
author_sort Perini, Mauro Alejandro
title Measurement of expansin activity and plant cell wall creep by using a commercial texture analyzer
title_short Measurement of expansin activity and plant cell wall creep by using a commercial texture analyzer
title_full Measurement of expansin activity and plant cell wall creep by using a commercial texture analyzer
title_fullStr Measurement of expansin activity and plant cell wall creep by using a commercial texture analyzer
title_full_unstemmed Measurement of expansin activity and plant cell wall creep by using a commercial texture analyzer
title_sort measurement of expansin activity and plant cell wall creep by using a commercial texture analyzer
publishDate 2017
url http://sedici.unlp.edu.ar/handle/10915/74669
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AT sinignacio measurementofexpansinactivityandplantcellwallcreepbyusingacommercialtextureanalyzer
AT martinezgustavoadolfo measurementofexpansinactivityandplantcellwallcreepbyusingacommercialtextureanalyzer
AT civellopedromarcos measurementofexpansinactivityandplantcellwallcreepbyusingacommercialtextureanalyzer
bdutipo_str Repositorios
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