Partial Molecular Characterization of Arctium minus Aspartylendopeptidase and Preparation of Bioactive Peptides by Whey Protein Hydrolysis

In this article, we report the cloning of an aspartic protease (AP) from flowers of Arctium minus (Hill) Bernh. (Asteraceae) along with the use of depigmented aqueous flower extracts, as a source of APs, for the hydrolysis of whey proteins. The isolated cDNA encoded a protein product with 509 amino...

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Detalles Bibliográficos
Autores principales:	Cimino, Cecilia Verónica, Colombo, María Laura, Liggieri, Constanza Silvina, Bruno, Mariela Anahí, Vairo Cavalli, Sandra Elizabeth
Formato:	Articulo
Lenguaje:	Inglés
Publicado:	2015
Materias:	Ciencias Exactas Biología angiotensin-converting enzyme, inhibitory activity, antioxidant capacity, arctiumisin, cloning, milk protein, typical plant aspartic protease
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/74527
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-74527
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Ciencias Exactas Biología angiotensin-converting enzyme, inhibitory activity, antioxidant capacity, arctiumisin, cloning, milk protein, typical plant aspartic protease
spellingShingle	Ciencias Exactas Biología angiotensin-converting enzyme, inhibitory activity, antioxidant capacity, arctiumisin, cloning, milk protein, typical plant aspartic protease Cimino, Cecilia Verónica Colombo, María Laura Liggieri, Constanza Silvina Bruno, Mariela Anahí Vairo Cavalli, Sandra Elizabeth Partial Molecular Characterization of Arctium minus Aspartylendopeptidase and Preparation of Bioactive Peptides by Whey Protein Hydrolysis
topic_facet	Ciencias Exactas Biología angiotensin-converting enzyme, inhibitory activity, antioxidant capacity, arctiumisin, cloning, milk protein, typical plant aspartic protease
description	In this article, we report the cloning of an aspartic protease (AP) from flowers of Arctium minus (Hill) Bernh. (Asteraceae) along with the use of depigmented aqueous flower extracts, as a source of APs, for the hydrolysis of whey proteins. The isolated cDNA encoded a protein product with 509 amino acids called arctiumisin, with the characteristic primary structure organization of typical plant APs. Bovine whey protein hydrolysates, obtained employing the enzyme extracts of A. minus flowers, displayed inhibitory angiotensin-converting enzyme (ACE) and antioxidant activities. Hydrolysates after 3 and 5 h of reaction (degree of hydrolysis 2.4 and 5.6, respectively) and the associated peptide fraction with molecular weight below 3 kDa were analyzed by sodium dodecyl sulfate–polyacrylamide gel electrophoresis, matrix-assisted laser desorption ionization/time of flight mass spectrometry, and reverse phase-high-performance liquid chromatography. The results obtained in this study demonstrate the viability of using proteases from A. minus to increase the antioxidant and inhibitory ACE capacity of whey proteins.
format	Articulo Articulo
author	Cimino, Cecilia Verónica Colombo, María Laura Liggieri, Constanza Silvina Bruno, Mariela Anahí Vairo Cavalli, Sandra Elizabeth
author_facet	Cimino, Cecilia Verónica Colombo, María Laura Liggieri, Constanza Silvina Bruno, Mariela Anahí Vairo Cavalli, Sandra Elizabeth
author_sort	Cimino, Cecilia Verónica
title	Partial Molecular Characterization of Arctium minus Aspartylendopeptidase and Preparation of Bioactive Peptides by Whey Protein Hydrolysis
title_short	Partial Molecular Characterization of Arctium minus Aspartylendopeptidase and Preparation of Bioactive Peptides by Whey Protein Hydrolysis
title_full	Partial Molecular Characterization of Arctium minus Aspartylendopeptidase and Preparation of Bioactive Peptides by Whey Protein Hydrolysis
title_fullStr	Partial Molecular Characterization of Arctium minus Aspartylendopeptidase and Preparation of Bioactive Peptides by Whey Protein Hydrolysis
title_full_unstemmed	Partial Molecular Characterization of Arctium minus Aspartylendopeptidase and Preparation of Bioactive Peptides by Whey Protein Hydrolysis
title_sort	partial molecular characterization of arctium minus aspartylendopeptidase and preparation of bioactive peptides by whey protein hydrolysis
publishDate	2015
url	http://sedici.unlp.edu.ar/handle/10915/74527
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Partial Molecular Characterization of Arctium minus Aspartylendopeptidase and Preparation of Bioactive Peptides by Whey Protein Hydrolysis

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