Vector Competence of Argentine Mosquitoes (Diptera: Culicidae) for West Nile virus (Flaviviridae: Flavivirus)

We examined the ability of Culex pipiens L. complex mosquitoes from Argentina to vector West Nile virus (WNV) to assess their role in the transmission of WNV in South America. Several egg rafts of Culex spp. were collected from different breeding sites in the suburbs of the city of La Plata, Argenti...

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Detalles Bibliográficos
Autores principales: Micieli, María Victoria, Matacchiero, Amy C., Muttis, Evangelina, Fonseca, Dina M., Aliota, Matthew T., Kramer, Laura D.
Formato: Articulo Preprint
Lenguaje:Inglés
Publicado: 2013
Materias:
Zoología
mosquitoes
arbovirus
West Nile virus
vector competence
Culicidae
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/73579
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-73579
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Zoología
mosquitoes
arbovirus
West Nile virus
vector competence
Culicidae
spellingShingle Zoología
mosquitoes
arbovirus
West Nile virus
vector competence
Culicidae
Micieli, María Victoria
Matacchiero, Amy C.
Muttis, Evangelina
Fonseca, Dina M.
Aliota, Matthew T.
Kramer, Laura D.
Vector Competence of Argentine Mosquitoes (Diptera: Culicidae) for West Nile virus (Flaviviridae: Flavivirus)
topic_facet Zoología
mosquitoes
arbovirus
West Nile virus
vector competence
Culicidae
description We examined the ability of Culex pipiens L. complex mosquitoes from Argentina to vector West Nile virus (WNV) to assess their role in the transmission of WNV in South America. Several egg rafts of Culex spp. were collected from different breeding sites in the suburbs of the city of La Plata, Argentina, and a subset of each progeny was scored with morphological and genetic species indicators. Surprisingly, we did not find Cx. pipiens form pipiens, but found evidence of genetic hybrids of Culex quinquefasciatus and Cx. pipiens f. molestus. We then used morphological traits to create two colonies predominantly composed of one of these two taxa, although some hybrids are likely to have been included in both. These colonies were used in vector competence studies using NY99 and WN02 genotype strains of WNV obtained in New York State. As controls, we also tested colonies of U.S. Cx. quinquefasciatus and Cx. pipiens f. molestus. Additional Culex larvae from three drainage ditches near the cities of La Plata and Berisso, Argentina, were identified by morphological and high-resolution molecular markers (microsatellites) as Cx. quinquefasciatus Say, Cx. pipiens form molestus, and hybrids. Results indicate that Argentinian Culex are competent but only moderately efficient vectors of WNV and are less susceptible to this virus than comparable U.S. mosquito strains. Studies of vertical transmission of NY99 virus by Cx. pipiens f. molestus hybrids from Argentina yielded a minimal filial infection rate of 1.19 from females feeding during their second and later bloodmeals.
format Articulo
Preprint
author Micieli, María Victoria
Matacchiero, Amy C.
Muttis, Evangelina
Fonseca, Dina M.
Aliota, Matthew T.
Kramer, Laura D.
author_facet Micieli, María Victoria
Matacchiero, Amy C.
Muttis, Evangelina
Fonseca, Dina M.
Aliota, Matthew T.
Kramer, Laura D.
author_sort Micieli, María Victoria
title Vector Competence of Argentine Mosquitoes (Diptera: Culicidae) for West Nile virus (Flaviviridae: Flavivirus)
title_short Vector Competence of Argentine Mosquitoes (Diptera: Culicidae) for West Nile virus (Flaviviridae: Flavivirus)
title_full Vector Competence of Argentine Mosquitoes (Diptera: Culicidae) for West Nile virus (Flaviviridae: Flavivirus)
title_fullStr Vector Competence of Argentine Mosquitoes (Diptera: Culicidae) for West Nile virus (Flaviviridae: Flavivirus)
title_full_unstemmed Vector Competence of Argentine Mosquitoes (Diptera: Culicidae) for West Nile virus (Flaviviridae: Flavivirus)
title_sort vector competence of argentine mosquitoes (diptera: culicidae) for west nile virus (flaviviridae: flavivirus)
publishDate 2013
url http://sedici.unlp.edu.ar/handle/10915/73579
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