Evaluation of apoptosis markers in different cell lines infected with equine arteritis virus

Equine arteritis virus (EAV) induces apoptosis in infected cells. Cell death caused by EAV has been studied mainly using three cell lines, BHK-21, RK-13 and Vero cells. The mechanism of apoptosis varies among cell lines and results cannot be correlated owing to differences in EAV strains used. We ev...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Metz, Germán Ernesto, Abeyá, María Mercedes, Serena, María Soledad, Panei, Carlos Javier, Echeverría, María Gabriela
Formato: Articulo Preprint
Lenguaje:Inglés
Publicado: 2018
Materias:
Ciencias Veterinarias
apoptosis; caspases; cell lines; DNA fragmentation; equine arteritis virus; flow cytometry
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/72216
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-72216
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Veterinarias
apoptosis; caspases; cell lines; DNA fragmentation; equine arteritis virus; flow cytometry
spellingShingle Ciencias Veterinarias
apoptosis; caspases; cell lines; DNA fragmentation; equine arteritis virus; flow cytometry
Metz, Germán Ernesto
Abeyá, María Mercedes
Serena, María Soledad
Panei, Carlos Javier
Echeverría, María Gabriela
Evaluation of apoptosis markers in different cell lines infected with equine arteritis virus
topic_facet Ciencias Veterinarias
apoptosis; caspases; cell lines; DNA fragmentation; equine arteritis virus; flow cytometry
description Equine arteritis virus (EAV) induces apoptosis in infected cells. Cell death caused by EAV has been studied mainly using three cell lines, BHK-21, RK-13 and Vero cells. The mechanism of apoptosis varies among cell lines and results cannot be correlated owing to differences in EAV strains used. We evaluated different markers for apoptosis in BHK-21, RK-13 and Vero cell lines using the Bucyrus EAV reference strain. Acridine orange/ethidium bromide staining revealed morphological 10 changes in infected cells, while flow cytometry indicated the extent of apoptosis. We also observed DNA fragmentation, but the DNA ladder was detected at different times post-infection depending on the cell line, i.e., 48, 72 and 96 h post-infection in RK-13, Vero and BHK-21 cells, respectively. Measurement of viral titers obtained with each cell line indicated that apoptosis causes interference with viral replication and therefore decreased viral titers. As an unequivocal 15 marker of apoptosis, we measured the expression of caspase-3 and caspases-8 and −9 as extrinsic and intrinsic markers of apoptosis pathways, respectively. Caspase-8 in BHK-21 cells was the only protease that was not detected at any of the times assayed. We found that Bucyrus EAV strain exhibited a distinctive apoptosis pathway depending on the cell line.
format Articulo
Preprint
author Metz, Germán Ernesto
Abeyá, María Mercedes
Serena, María Soledad
Panei, Carlos Javier
Echeverría, María Gabriela
author_facet Metz, Germán Ernesto
Abeyá, María Mercedes
Serena, María Soledad
Panei, Carlos Javier
Echeverría, María Gabriela
author_sort Metz, Germán Ernesto
title Evaluation of apoptosis markers in different cell lines infected with equine arteritis virus
title_short Evaluation of apoptosis markers in different cell lines infected with equine arteritis virus
title_full Evaluation of apoptosis markers in different cell lines infected with equine arteritis virus
title_fullStr Evaluation of apoptosis markers in different cell lines infected with equine arteritis virus
title_full_unstemmed Evaluation of apoptosis markers in different cell lines infected with equine arteritis virus
title_sort evaluation of apoptosis markers in different cell lines infected with equine arteritis virus
publishDate 2018
url http://sedici.unlp.edu.ar/handle/10915/72216
work_keys_str_mv AT metzgermanernesto evaluationofapoptosismarkersindifferentcelllinesinfectedwithequinearteritisvirus
AT abeyamariamercedes evaluationofapoptosismarkersindifferentcelllinesinfectedwithequinearteritisvirus
AT serenamariasoledad evaluationofapoptosismarkersindifferentcelllinesinfectedwithequinearteritisvirus
AT paneicarlosjavier evaluationofapoptosismarkersindifferentcelllinesinfectedwithequinearteritisvirus
AT echeverriamariagabriela evaluationofapoptosismarkersindifferentcelllinesinfectedwithequinearteritisvirus
bdutipo_str Repositorios
_version_ 1764820483561226242

Ejemplares similares