Chlorhexidine delivery system from titanium/polybenzyl acrylate coating: Evaluation of cytotoxicity and early bacterial adhesion

Objectives: The formation of biofilms on titanium dental implants is one of the main causes of failure of these devices. Streptococci are considered early colonizers that alter local environment favouring growing conditions for other colonizers. Chlorhexidine (CHX) is so far the most effective antim...

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Autores principales: Cortizo, María Cecilia, Oberti, Tamara Gisela, Cortizo, María Susana, Cortizo, Ana María, Fernández Lorenzo de Mele, Mónica Alicia
Formato: Articulo
Lenguaje:Inglés
Publicado: 2012
Materias:
Ciencias Exactas
Odontología
titanium dental implant, acrylate chlorhexidine, biocompatibility testing, bacterial adhesion
Implantes Dentales
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/71082
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-71082
record_format dspace
spelling I19-R120-10915-710822023-12-04T14:59:17Z http://sedici.unlp.edu.ar/handle/10915/71082 Chlorhexidine delivery system from titanium/polybenzyl acrylate coating: Evaluation of cytotoxicity and early bacterial adhesion Cortizo, María Cecilia Oberti, Tamara Gisela Cortizo, María Susana Cortizo, Ana María Fernández Lorenzo de Mele, Mónica Alicia 2012 2018-11-29T15:14:59Z en Ciencias Exactas Odontología titanium dental implant, acrylate chlorhexidine, biocompatibility testing, bacterial adhesion Implantes Dentales Objectives: The formation of biofilms on titanium dental implants is one of the main causes of failure of these devices. Streptococci are considered early colonizers that alter local environment favouring growing conditions for other colonizers. Chlorhexidine (CHX) is so far the most effective antimicrobial treatment against a wide variety of Gram-positive and Gram-negative organisms as well as fungi. This study was designed to develop a CHX delivery system appropriate for healing caps and abutments, with suitable drug release rate, effective as antimicrobial agent, and free of cytotoxic effects. Methods: Polybenzyl acrylate (PBA) coatings with and without CHX (Ti/PBA and Ti/PBA-CHX, respectively) and different drug loads (0.35, 0.70, and 1.40%, w/w) were assayed. The cytotoxic effect of CHX released from the different substrates on UMR106 cells was tested by alkaline phosphatase specific activity (ALP), and microscopic evaluation of the cells. Noncytotoxic drug load (0.35%, w/w) was selected to evaluate the antimicrobial effectiveness of the system using a microbial consortium of Streptococcus species. Results: The kinetic profile of CHX delivered by Ti/PBA-CHX showed an initial fast release rate followed by a monotonic increase of delivered mass over 48 h. The number of attached bacteria decreased in the following order: Ti > Ti/PBA > Ti/PBA-0.35. Conclusions: PBA-0.35 coating is effective to inhibit the adhesion of early colonizers on Ti without any cytotoxic effect on UMR-106 cells. Facultad de Ciencias Exactas Articulo Articulo http://creativecommons.org/licenses/by/4.0/ Creative Commons Attribution 4.0 International (CC BY 4.0) application/pdf 329-337
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
Odontología
titanium dental implant, acrylate chlorhexidine, biocompatibility testing, bacterial adhesion
Implantes Dentales
spellingShingle Ciencias Exactas
Odontología
titanium dental implant, acrylate chlorhexidine, biocompatibility testing, bacterial adhesion
Implantes Dentales
Cortizo, María Cecilia
Oberti, Tamara Gisela
Cortizo, María Susana
Cortizo, Ana María
Fernández Lorenzo de Mele, Mónica Alicia
Chlorhexidine delivery system from titanium/polybenzyl acrylate coating: Evaluation of cytotoxicity and early bacterial adhesion
topic_facet Ciencias Exactas
Odontología
titanium dental implant, acrylate chlorhexidine, biocompatibility testing, bacterial adhesion
Implantes Dentales
description Objectives: The formation of biofilms on titanium dental implants is one of the main causes of failure of these devices. Streptococci are considered early colonizers that alter local environment favouring growing conditions for other colonizers. Chlorhexidine (CHX) is so far the most effective antimicrobial treatment against a wide variety of Gram-positive and Gram-negative organisms as well as fungi. This study was designed to develop a CHX delivery system appropriate for healing caps and abutments, with suitable drug release rate, effective as antimicrobial agent, and free of cytotoxic effects. Methods: Polybenzyl acrylate (PBA) coatings with and without CHX (Ti/PBA and Ti/PBA-CHX, respectively) and different drug loads (0.35, 0.70, and 1.40%, w/w) were assayed. The cytotoxic effect of CHX released from the different substrates on UMR106 cells was tested by alkaline phosphatase specific activity (ALP), and microscopic evaluation of the cells. Noncytotoxic drug load (0.35%, w/w) was selected to evaluate the antimicrobial effectiveness of the system using a microbial consortium of Streptococcus species. Results: The kinetic profile of CHX delivered by Ti/PBA-CHX showed an initial fast release rate followed by a monotonic increase of delivered mass over 48 h. The number of attached bacteria decreased in the following order: Ti > Ti/PBA > Ti/PBA-0.35. Conclusions: PBA-0.35 coating is effective to inhibit the adhesion of early colonizers on Ti without any cytotoxic effect on UMR-106 cells.
format Articulo
Articulo
author Cortizo, María Cecilia
Oberti, Tamara Gisela
Cortizo, María Susana
Cortizo, Ana María
Fernández Lorenzo de Mele, Mónica Alicia
author_facet Cortizo, María Cecilia
Oberti, Tamara Gisela
Cortizo, María Susana
Cortizo, Ana María
Fernández Lorenzo de Mele, Mónica Alicia
author_sort Cortizo, María Cecilia
title Chlorhexidine delivery system from titanium/polybenzyl acrylate coating: Evaluation of cytotoxicity and early bacterial adhesion
title_short Chlorhexidine delivery system from titanium/polybenzyl acrylate coating: Evaluation of cytotoxicity and early bacterial adhesion
title_full Chlorhexidine delivery system from titanium/polybenzyl acrylate coating: Evaluation of cytotoxicity and early bacterial adhesion
title_fullStr Chlorhexidine delivery system from titanium/polybenzyl acrylate coating: Evaluation of cytotoxicity and early bacterial adhesion
title_full_unstemmed Chlorhexidine delivery system from titanium/polybenzyl acrylate coating: Evaluation of cytotoxicity and early bacterial adhesion
title_sort chlorhexidine delivery system from titanium/polybenzyl acrylate coating: evaluation of cytotoxicity and early bacterial adhesion
publishDate 2012
url http://sedici.unlp.edu.ar/handle/10915/71082
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