Biomarcadores hepáticos de la carpa (<i>Cyprinus carpio</i> L.): respuestas antioxidantes inducidas por la β-naftoflavona, un hidrocarburo aromático policíclico

Polycyclic aromatic hydrocarbons (PAHs) exert adverse effects in many aquatic organisms and fish among them. β-naphtoflavone (BNF) is a PAH inductor of biotransformation processes that potentially could enhance the production of reactive oxygen species promoting the responses of the cellular antioxi...

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Detalles Bibliográficos
Autores principales:	Ascar, M. I., De la Torre, F. R.
Formato:	Articulo
Lenguaje:	Español
Publicado:	2006
Materias:	Ciencias Naturales Hidrocarburos Policíclicos Aromáticos Contaminación Costera biomarkers of contamination, antioxidant defences, PAHs, Cyprinus carpio
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/69258
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-69258
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Español
topic	Ciencias Naturales Hidrocarburos Policíclicos Aromáticos Contaminación Costera biomarkers of contamination, antioxidant defences, PAHs, Cyprinus carpio
spellingShingle	Ciencias Naturales Hidrocarburos Policíclicos Aromáticos Contaminación Costera biomarkers of contamination, antioxidant defences, PAHs, Cyprinus carpio Ascar, M. I. De la Torre, F. R. Biomarcadores hepáticos de la carpa (<i>Cyprinus carpio</i> L.): respuestas antioxidantes inducidas por la β-naftoflavona, un hidrocarburo aromático policíclico
topic_facet	Ciencias Naturales Hidrocarburos Policíclicos Aromáticos Contaminación Costera biomarkers of contamination, antioxidant defences, PAHs, Cyprinus carpio
description	Polycyclic aromatic hydrocarbons (PAHs) exert adverse effects in many aquatic organisms and fish among them. β-naphtoflavone (BNF) is a PAH inductor of biotransformation processes that potentially could enhance the production of reactive oxygen species promoting the responses of the cellular antioxidant defences. Hepatic biomarker responses were assessed in juvenile Cyprinus carpio injected with a sublethal dose of BNF; catalase (CAT), Superoxid dismutase (SOD),Glutathion S-transferase (GST) activities, the condition factor (FC) and the liver somatic index (IHS) were evaluated. Fish were acclimated to experimental conditions for 1 week (continuous flow potable water: 50 ml/ min; 2,5 g fish/L; photoperiod: 12 h L/D; temperature: 22 ºC; daily feeding ad libitum). At the beginning of the assays experimental fish (BNF) (n=10) were intraperitoneally injected with 50 mg BNF/kg. b. w. dissolved in corn oil; controls (C) (n=10) received corn oil. No fish mortality was observed along the assay. After 48 h, fish were killed, livers excised and FC and IHS index were determined. Then, tissues were homogenized and postmitoncondrial fractions were obtained; total protein content, and specific activities of CAT, SOD and GST were determined in this fraction. Statistical differences between groups C and BNF were performed by Student´s t test (p<0.05). A significant increase of GST (22.8%) and SOD (27.3%) activities were observed in BNF fish, but no differences were detected in CAT suggesting there is an effective capacity of SOD in removing the (O2 -) of the cellular media; the absence of differences in CAT would indicate that the formed H2O2 was only partially catalyzed. No statistical differences were observed in the IHS and FC indexes. These results will also allow considering the use of these valuable environmental bioindicators of this test species in the assessment of the quality of water bodies of the Buenos Aires Province.
format	Articulo Articulo
author	Ascar, M. I. De la Torre, F. R.
author_facet	Ascar, M. I. De la Torre, F. R.
author_sort	Ascar, M. I.
title	Biomarcadores hepáticos de la carpa (<i>Cyprinus carpio</i> L.): respuestas antioxidantes inducidas por la β-naftoflavona, un hidrocarburo aromático policíclico
title_short	Biomarcadores hepáticos de la carpa (<i>Cyprinus carpio</i> L.): respuestas antioxidantes inducidas por la β-naftoflavona, un hidrocarburo aromático policíclico
title_full	Biomarcadores hepáticos de la carpa (<i>Cyprinus carpio</i> L.): respuestas antioxidantes inducidas por la β-naftoflavona, un hidrocarburo aromático policíclico
title_fullStr	Biomarcadores hepáticos de la carpa (<i>Cyprinus carpio</i> L.): respuestas antioxidantes inducidas por la β-naftoflavona, un hidrocarburo aromático policíclico
title_full_unstemmed	Biomarcadores hepáticos de la carpa (<i>Cyprinus carpio</i> L.): respuestas antioxidantes inducidas por la β-naftoflavona, un hidrocarburo aromático policíclico
title_sort	biomarcadores hepáticos de la carpa (<i>cyprinus carpio</i> l.): respuestas antioxidantes inducidas por la β-naftoflavona, un hidrocarburo aromático policíclico
publishDate	2006
url	http://sedici.unlp.edu.ar/handle/10915/69258
work_keys_str_mv	AT ascarmi biomarcadoreshepaticosdelacarpaicyprinuscarpioilrespuestasantioxidantesinducidasporlabnaftoflavonaunhidrocarburoaromaticopoliciclico AT delatorrefr biomarcadoreshepaticosdelacarpaicyprinuscarpioilrespuestasantioxidantesinducidasporlabnaftoflavonaunhidrocarburoaromaticopoliciclico
bdutipo_str	Repositorios
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Biomarcadores hepáticos de la carpa (<i>Cyprinus carpio</i> L.): respuestas antioxidantes inducidas por la β-naftoflavona, un hidrocarburo aromático policíclico

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