Expression of M Protein from LP02/C Equine Arteritis Virus Inhibits Growth of <i>Escherichia Coli</i> M15-pQE30 System

Our objective was to obtain Equine Arteritis Virus M protein in prokaryotic system to test it as immunogen. LP02/C Equine Arteritis Virus cDNA was used as template to obtain and clone this protein. Equine Arteritis M protein was cloned in the expression vector pQE30 and the recom...

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Autores principales: Metz, Germán Ernesto, Abeyá, María Mercedes, Serena, María Soledad, Panei, Carlos Javier, Díaz, Silvina, Echeverría, María Gabriela
Formato: Articulo
Lenguaje:Inglés
Publicado: 2017
Materias:
Ciencias Veterinarias
equine arteritis virus
M protein
equinos
virus
Escherichia coli M15
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/68168
http://www.oatext.com/Expression-of-M-Protein-from-LP02C-Equine-Arteritis-Virus-Inhibits-Growth-of-Escherichia-Coli-M15-Pqe30-System.php
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SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-68168
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Veterinarias
equine arteritis virus
M protein
equinos
virus
Escherichia coli M15
spellingShingle Ciencias Veterinarias
equine arteritis virus
M protein
equinos
virus
Escherichia coli M15
Metz, Germán Ernesto
Abeyá, María Mercedes
Serena, María Soledad
Panei, Carlos Javier
Díaz, Silvina
Echeverría, María Gabriela
Expression of M Protein from LP02/C Equine Arteritis Virus Inhibits Growth of <i>Escherichia Coli</i> M15-pQE30 System
topic_facet Ciencias Veterinarias
equine arteritis virus
M protein
equinos
virus
Escherichia coli M15
description Our objective was to obtain Equine Arteritis Virus M protein in prokaryotic system to test it as immunogen. LP02/C Equine Arteritis Virus cDNA was used as template to obtain and clone this protein. Equine Arteritis M protein was cloned in the expression vector pQE30 and the recombinant plasmid pQE30/M was transformed in Escherichia Coli M15 cells. The OD600 values of the IPTG-induced M15-pQE30/M culture showed an inhibition of the kinetics growth compared with the non-induced M15-pQE30/M and positive M15-pQE40/DHFR cultures. Several factors such as growth temperature, IPTG concentration and different inductors were analyzed but any of them showed an improvement in protein expression. Instead of E. coli M15strain, a new strain (E. coli BL21) was used and transformed with the pQE30/M. This resolved in part the growth inhibition observed in E. coli M15 cells, but no the recovery yield of the protein. So, as all gene products that affect cells kinetics growth are considered to be toxic, we argue that the lower yields in M protein recovery could be attributed to an associated toxicity of EAV-M protein from LP02/C strain in this expression system.
format Articulo
Articulo
author Metz, Germán Ernesto
Abeyá, María Mercedes
Serena, María Soledad
Panei, Carlos Javier
Díaz, Silvina
Echeverría, María Gabriela
author_facet Metz, Germán Ernesto
Abeyá, María Mercedes
Serena, María Soledad
Panei, Carlos Javier
Díaz, Silvina
Echeverría, María Gabriela
author_sort Metz, Germán Ernesto
title Expression of M Protein from LP02/C Equine Arteritis Virus Inhibits Growth of <i>Escherichia Coli</i> M15-pQE30 System
title_short Expression of M Protein from LP02/C Equine Arteritis Virus Inhibits Growth of <i>Escherichia Coli</i> M15-pQE30 System
title_full Expression of M Protein from LP02/C Equine Arteritis Virus Inhibits Growth of <i>Escherichia Coli</i> M15-pQE30 System
title_fullStr Expression of M Protein from LP02/C Equine Arteritis Virus Inhibits Growth of <i>Escherichia Coli</i> M15-pQE30 System
title_full_unstemmed Expression of M Protein from LP02/C Equine Arteritis Virus Inhibits Growth of <i>Escherichia Coli</i> M15-pQE30 System
title_sort expression of m protein from lp02/c equine arteritis virus inhibits growth of <i>escherichia coli</i> m15-pqe30 system
publishDate 2017
url http://sedici.unlp.edu.ar/handle/10915/68168
http://www.oatext.com/Expression-of-M-Protein-from-LP02C-Equine-Arteritis-Virus-Inhibits-Growth-of-Escherichia-Coli-M15-Pqe30-System.php
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