Immunization with the Recombinant Cholera Toxin B Fused to Fimbria 2 Protein Protects against <i>Bordetella pertussis</i> Infection

This study examined the immunogenic properties of the fusion protein fimbria 2 of Bordetella pertussis (Fim2)—cholera toxin B subunit (CTB) in the intranasal murine model of infection. To this end B. pertussis Fim2 coding sequence was cloned downstream of the cholera toxin B subunit coding sequence....

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Autores principales: Olivera, María Noelia, Castuma, Celina E., Hozbor, Daniela Flavia, Gaillard, María Emilia, Rumbo, Martín, Gómez, Ricardo Martín
Formato: Articulo
Lenguaje:Inglés
Publicado: 2014
Materias:
Ciencias Exactas
Bordetella pertussis
Inmunización
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/68057
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-68057
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
Bordetella pertussis
Inmunización
spellingShingle Ciencias Exactas
Bordetella pertussis
Inmunización
Olivera, María Noelia
Castuma, Celina E.
Hozbor, Daniela Flavia
Gaillard, María Emilia
Rumbo, Martín
Gómez, Ricardo Martín
Immunization with the Recombinant Cholera Toxin B Fused to Fimbria 2 Protein Protects against <i>Bordetella pertussis</i> Infection
topic_facet Ciencias Exactas
Bordetella pertussis
Inmunización
description This study examined the immunogenic properties of the fusion protein fimbria 2 of Bordetella pertussis (Fim2)—cholera toxin B subunit (CTB) in the intranasal murine model of infection. To this end B. pertussis Fim2 coding sequence was cloned downstream of the cholera toxin B subunit coding sequence.Theexpression and assembly of the fusion protein into pentameric structures (CTBFim2) were evaluated by SDS-PAGE and monosialotetrahexosylgaglioside (GM1-ganglioside) enzyme-linked immunosorbent assay (ELISA). To evaluate the protective capacity of CTB-Fim2, an intraperitoneal or intranasal mouse immunization schedule was performed with 50 μg of CTB-Fim2. Recombinant (rFim2) or purified (BpFim2) Fim2, CTB, and phosphate-buffered saline (PBS) were used as controls. The results showed that mice immunized with BpFim2 or CTB-Fim2 intraperitoneally or intranasally presented a significant reduction in bacterial lung counts compared to control groups (P < 0.01 or P < 0.001, resp.). Moreover, intranasal immunization with CTB-Fim2 induced significant levels of Fim2-specific IgG in serum and bronchoalveolar lavage (BAL) and Fim2-specific IgA in BAL. Analysis of IgG isotypes and cytokines mRNA levels showed that CTB-Fim2 results in a mixed Th1/Th2 (T-helper) response. The data presented here provide support for CTB-Fim2 as a promising recombinant antigen against Bordetella pertussis infection.
format Articulo
Articulo
author Olivera, María Noelia
Castuma, Celina E.
Hozbor, Daniela Flavia
Gaillard, María Emilia
Rumbo, Martín
Gómez, Ricardo Martín
author_facet Olivera, María Noelia
Castuma, Celina E.
Hozbor, Daniela Flavia
Gaillard, María Emilia
Rumbo, Martín
Gómez, Ricardo Martín
author_sort Olivera, María Noelia
title Immunization with the Recombinant Cholera Toxin B Fused to Fimbria 2 Protein Protects against <i>Bordetella pertussis</i> Infection
title_short Immunization with the Recombinant Cholera Toxin B Fused to Fimbria 2 Protein Protects against <i>Bordetella pertussis</i> Infection
title_full Immunization with the Recombinant Cholera Toxin B Fused to Fimbria 2 Protein Protects against <i>Bordetella pertussis</i> Infection
title_fullStr Immunization with the Recombinant Cholera Toxin B Fused to Fimbria 2 Protein Protects against <i>Bordetella pertussis</i> Infection
title_full_unstemmed Immunization with the Recombinant Cholera Toxin B Fused to Fimbria 2 Protein Protects against <i>Bordetella pertussis</i> Infection
title_sort immunization with the recombinant cholera toxin b fused to fimbria 2 protein protects against <i>bordetella pertussis</i> infection
publishDate 2014
url http://sedici.unlp.edu.ar/handle/10915/68057
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