Biochemical and MALDI-TOF Mass Spectrometric Characterization of a Novel Native and Recombinant Cystine Knot Miniprotein from <i>Solanum tuberosum</i> subsp. <i>andigenum</i> cv. Churqueña

Cystine-knot miniproteins (CKMPs) are an intriguing group of cysteine-rich molecules that combine the characteristics of proteins and peptides. Typically, CKMPs are fewer than 50 residues in length and share a characteristic knotted scaffold characterized by the presence of three intramolecular disu...

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Detalles Bibliográficos
Autores principales: Cotabarren, Juliana, Tellechea, Mariana Edith, Tanco, Sebastián Martín, Lorenzo, Julia, García Pardo, Javier, Avilés, Francesc Xavier, Obregón, Walter David
Formato: Articulo
Lenguaje:Inglés
Publicado: 2018
Materias:
Biología
cystine-knot miniproteins; carboxypeptidase inhibitor; plant inhibitor; Solanum tuberosum; protease; Andean potatoes
Cistina
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/68045
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-68045
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Biología
cystine-knot miniproteins; carboxypeptidase inhibitor; plant inhibitor; Solanum tuberosum; protease; Andean potatoes
Cistina
spellingShingle Biología
cystine-knot miniproteins; carboxypeptidase inhibitor; plant inhibitor; Solanum tuberosum; protease; Andean potatoes
Cistina
Cotabarren, Juliana
Tellechea, Mariana Edith
Tanco, Sebastián Martín
Lorenzo, Julia
García Pardo, Javier
Avilés, Francesc Xavier
Obregón, Walter David
Biochemical and MALDI-TOF Mass Spectrometric Characterization of a Novel Native and Recombinant Cystine Knot Miniprotein from <i>Solanum tuberosum</i> subsp. <i>andigenum</i> cv. Churqueña
topic_facet Biología
cystine-knot miniproteins; carboxypeptidase inhibitor; plant inhibitor; Solanum tuberosum; protease; Andean potatoes
Cistina
description Cystine-knot miniproteins (CKMPs) are an intriguing group of cysteine-rich molecules that combine the characteristics of proteins and peptides. Typically, CKMPs are fewer than 50 residues in length and share a characteristic knotted scaffold characterized by the presence of three intramolecular disulfide bonds that form the singular knotted structure. The knot scaffold confers on these proteins remarkable chemical, thermal, and proteolytic stability. Recently, CKMPs have emerged as a novel class of natural molecules with interesting pharmacological properties. In the present work, a novel cystine-knot metallocarboxypeptidase inhibitor (chuPCI) was isolated from tubers of Solanum tuberosum, subsp. andigenum cv. Churqueña. Our results demonstrated that chuPCI is a member of the A/B-type family of metallocarboxypeptidases inhibitors. chuPCI was expressed and characterized by a combination of biochemical and mass spectrometric techniques. Direct comparison of the MALDI-TOF mass spectra for the native and recombinant molecules allowed us to confirm the presence of four different forms of chuPCI in the tubers. The majority of such forms have a molecular weight of 4309 Da and contain a cyclized Gln in the N-terminus. The other three forms are derived from N-terminal and/or C-terminal proteolytic cleavages. Taken together, our results contribute to increase the current repertoire of natural CKMPs.
format Articulo
Articulo
author Cotabarren, Juliana
Tellechea, Mariana Edith
Tanco, Sebastián Martín
Lorenzo, Julia
García Pardo, Javier
Avilés, Francesc Xavier
Obregón, Walter David
author_facet Cotabarren, Juliana
Tellechea, Mariana Edith
Tanco, Sebastián Martín
Lorenzo, Julia
García Pardo, Javier
Avilés, Francesc Xavier
Obregón, Walter David
author_sort Cotabarren, Juliana
title Biochemical and MALDI-TOF Mass Spectrometric Characterization of a Novel Native and Recombinant Cystine Knot Miniprotein from <i>Solanum tuberosum</i> subsp. <i>andigenum</i> cv. Churqueña
title_short Biochemical and MALDI-TOF Mass Spectrometric Characterization of a Novel Native and Recombinant Cystine Knot Miniprotein from <i>Solanum tuberosum</i> subsp. <i>andigenum</i> cv. Churqueña
title_full Biochemical and MALDI-TOF Mass Spectrometric Characterization of a Novel Native and Recombinant Cystine Knot Miniprotein from <i>Solanum tuberosum</i> subsp. <i>andigenum</i> cv. Churqueña
title_fullStr Biochemical and MALDI-TOF Mass Spectrometric Characterization of a Novel Native and Recombinant Cystine Knot Miniprotein from <i>Solanum tuberosum</i> subsp. <i>andigenum</i> cv. Churqueña
title_full_unstemmed Biochemical and MALDI-TOF Mass Spectrometric Characterization of a Novel Native and Recombinant Cystine Knot Miniprotein from <i>Solanum tuberosum</i> subsp. <i>andigenum</i> cv. Churqueña
title_sort biochemical and maldi-tof mass spectrometric characterization of a novel native and recombinant cystine knot miniprotein from <i>solanum tuberosum</i> subsp. <i>andigenum</i> cv. churqueña
publishDate 2018
url http://sedici.unlp.edu.ar/handle/10915/68045
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