Callus culture for biomass production of milk thistle as a potential source of milk clotting peptidases

The objective of this work was the optimization of the conditions of in vitro culture for callus production of Silybum marianum (L.) Gaertn. (Asteraceae). Sections of cotyledons, previously disinfected by washing successively with ethanol 70º, NaClO (10% w/v) and Tween 20 (0.05% v/v) and rinsing wit...

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Detalles Bibliográficos
Autores principales:	Cimino, Cecilia Verónica, Vairo Cavalli, Sandra Elizabeth, Spina, Francisco, Natalucci, Claudia Luisa, Priolo de Lufrano, Nora Silvia
Formato:	Articulo
Lenguaje:	Español
Publicado:	2006
Materias:	Ciencias Exactas Asteraceae, callus, rhizogenesis, Silybum marianum Asteraceae peptidasas
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/67027 http://www.ejbiotechnology.info/index.php/ejbiotechnology/article/view/v9n3-14/280
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-67027
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Español
topic	Ciencias Exactas Asteraceae, callus, rhizogenesis, Silybum marianum Asteraceae peptidasas
spellingShingle	Ciencias Exactas Asteraceae, callus, rhizogenesis, Silybum marianum Asteraceae peptidasas Cimino, Cecilia Verónica Vairo Cavalli, Sandra Elizabeth Spina, Francisco Natalucci, Claudia Luisa Priolo de Lufrano, Nora Silvia Callus culture for biomass production of milk thistle as a potential source of milk clotting peptidases
topic_facet	Ciencias Exactas Asteraceae, callus, rhizogenesis, Silybum marianum Asteraceae peptidasas
description	The objective of this work was the optimization of the conditions of in vitro culture for callus production of Silybum marianum (L.) Gaertn. (Asteraceae). Sections of cotyledons, previously disinfected by washing successively with ethanol 70º, NaClO (10% w/v) and Tween 20 (0.05% v/v) and rinsing with sterile distilled water, were used as explants. For its initial culture, B5 medium supplemented with BA and 2,4-D solidified with phytagel was used, and a 63% survival was achieved. To obtain callus, two solid media were assayed (S1 and S2) using B5 medium supplemented with growth regulators (BA and 2,4-D or NAA and BA, respectively). The calli were grown at 25ºC during 45 days in darkness. Growth kinetics was studied using S1 medium obtaining a typical growth curve with an exponential phase after 14 days of incubation (rate of growth 0.005 g dry weight/ day) and stationary phase after 35 days. The rate of growth in S2 medium was slower, and rhizogenesis was observed starting on the fifth week of incubation. From these results, the best culture medium for callus production of Silybum marianum was S1 medium.
format	Articulo Articulo
author	Cimino, Cecilia Verónica Vairo Cavalli, Sandra Elizabeth Spina, Francisco Natalucci, Claudia Luisa Priolo de Lufrano, Nora Silvia
author_facet	Cimino, Cecilia Verónica Vairo Cavalli, Sandra Elizabeth Spina, Francisco Natalucci, Claudia Luisa Priolo de Lufrano, Nora Silvia
author_sort	Cimino, Cecilia Verónica
title	Callus culture for biomass production of milk thistle as a potential source of milk clotting peptidases
title_short	Callus culture for biomass production of milk thistle as a potential source of milk clotting peptidases
title_full	Callus culture for biomass production of milk thistle as a potential source of milk clotting peptidases
title_fullStr	Callus culture for biomass production of milk thistle as a potential source of milk clotting peptidases
title_full_unstemmed	Callus culture for biomass production of milk thistle as a potential source of milk clotting peptidases
title_sort	callus culture for biomass production of milk thistle as a potential source of milk clotting peptidases
publishDate	2006
url	http://sedici.unlp.edu.ar/handle/10915/67027 http://www.ejbiotechnology.info/index.php/ejbiotechnology/article/view/v9n3-14/280
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bdutipo_str	Repositorios
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Callus culture for biomass production of milk thistle as a potential source of milk clotting peptidases

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