In vitro interaction between <i>Bacillus megaterium</i> strains and Caco-2 cells

To further our understanding of the virulence potential of Bacillus megaterium strains, cell association and invasion assays were conducted in vitro by infecting human enterocytes (Caco-2 cells) with 53 strains of this bacterium isolated from honey. Two series of experiments were performed: (i) necr...

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Autores principales: López, Ana Claudia, Minnaard, Jessica, Pérez, Pablo Fernando, Alippi, Adriana Mónica
Formato: Articulo
Lenguaje:Inglés
Publicado: 2013
Materias:
Ciencias Agrarias
Bacterias
Bacillus megaterium; Caco-2 cells; adhesion; cellular necrosis; spent culture supernatant; honey
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/66801
http://revistes.iec.cat/index.php/IM/article/viewFile/67274/67058
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-66801
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Agrarias
Bacterias
Bacillus megaterium; Caco-2 cells; adhesion; cellular necrosis; spent culture supernatant; honey
spellingShingle Ciencias Agrarias
Bacterias
Bacillus megaterium; Caco-2 cells; adhesion; cellular necrosis; spent culture supernatant; honey
López, Ana Claudia
Minnaard, Jessica
Pérez, Pablo Fernando
Alippi, Adriana Mónica
In vitro interaction between <i>Bacillus megaterium</i> strains and Caco-2 cells
topic_facet Ciencias Agrarias
Bacterias
Bacillus megaterium; Caco-2 cells; adhesion; cellular necrosis; spent culture supernatant; honey
description To further our understanding of the virulence potential of Bacillus megaterium strains, cell association and invasion assays were conducted in vitro by infecting human enterocytes (Caco-2 cells) with 53 strains of this bacterium isolated from honey. Two series of experiments were performed: (i) necrosis and cell detachment assays with the supernatants of bacterial culture fi ltrates from 16-h cultures and (ii) adhesion/invasion assays in which cultured enterocytes incubated with bacteria from 3-h cultures were resuspended in Dulbecco’s modifi ed Eagle’s medium and chloramphenicol. The detachment of Caco-2 cells was evaluated by staining the cells with crystal violet. Necrosis was assessed by fl uorescence microscopy of cells labeled with propidium iodide. Association (adhesion plus invasion) was determined by plate counts and invasion in an aminoglycoside protection assay. The results showed that spent culture supernatants detached and necrotized Caco-2 cells in a strain-dependent manner. Seven out of 53 B. megaterium fi ltered culture supernatants caused complete cell detachment. Suspensions of these same bacterial strains adhered and invaded enterocytes in 2-h infection experiments. To our knowledge, this is the fi rst report on the interaction between B. megaterium and intestinal epithelial Caco-2 cells.
format Articulo
Articulo
author López, Ana Claudia
Minnaard, Jessica
Pérez, Pablo Fernando
Alippi, Adriana Mónica
author_facet López, Ana Claudia
Minnaard, Jessica
Pérez, Pablo Fernando
Alippi, Adriana Mónica
author_sort López, Ana Claudia
title In vitro interaction between <i>Bacillus megaterium</i> strains and Caco-2 cells
title_short In vitro interaction between <i>Bacillus megaterium</i> strains and Caco-2 cells
title_full In vitro interaction between <i>Bacillus megaterium</i> strains and Caco-2 cells
title_fullStr In vitro interaction between <i>Bacillus megaterium</i> strains and Caco-2 cells
title_full_unstemmed In vitro interaction between <i>Bacillus megaterium</i> strains and Caco-2 cells
title_sort in vitro interaction between <i>bacillus megaterium</i> strains and caco-2 cells
publishDate 2013
url http://sedici.unlp.edu.ar/handle/10915/66801
http://revistes.iec.cat/index.php/IM/article/viewFile/67274/67058
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AT minnaardjessica invitrointeractionbetweenibacillusmegateriumistrainsandcaco2cells
AT perezpablofernando invitrointeractionbetweenibacillusmegateriumistrainsandcaco2cells
AT alippiadrianamonica invitrointeractionbetweenibacillusmegateriumistrainsandcaco2cells
bdutipo_str Repositorios
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