Draft Genome Sequence of the Plant-Pathogenic Fungus <i>Stemphylium lycopersici</i> Strain CIDEFI-216

Stemphylium lycopersiciis a pathogenic fungus that provokes the leaf spot disease on over 30 host genera worldwide, among them Tomato. Here we report the draft genome sequence ofS. lycopersicistrain CIDEFI-216. Total genomic DNA was isolated from a monosporic culture using the DNeasy Plant Mini Kit...

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Detalles Bibliográficos
Autores principales: Franco, Mario Emilio Ernesto, López, Silvina Marianela Yanil, Medina, Rocío, Saparrat, Mario Carlos Nazareno, Balatti, Pedro Alberto
Formato: Objeto de conferencia
Lenguaje:Inglés
Publicado: 2015
Materias:
Ciencias Agrarias
Enfermedades de las Plantas
Hongos
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/66623
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-66623
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Agrarias
Enfermedades de las Plantas
Hongos
spellingShingle Ciencias Agrarias
Enfermedades de las Plantas
Hongos
Franco, Mario Emilio Ernesto
López, Silvina Marianela Yanil
Medina, Rocío
Saparrat, Mario Carlos Nazareno
Balatti, Pedro Alberto
Draft Genome Sequence of the Plant-Pathogenic Fungus <i>Stemphylium lycopersici</i> Strain CIDEFI-216
topic_facet Ciencias Agrarias
Enfermedades de las Plantas
Hongos
description Stemphylium lycopersiciis a pathogenic fungus that provokes the leaf spot disease on over 30 host genera worldwide, among them Tomato. Here we report the draft genome sequence ofS. lycopersicistrain CIDEFI-216. Total genomic DNA was isolated from a monosporic culture using the DNeasy Plant Mini Kit (Qiagen) and was used to construct 100 bp paired-end libraries, which were then sequenced by Illumina HiSeq 2000 technology. Reads were assembled using SOAPdenovo2 software at Macrogen (Korea).Gene prediction was performed by Fgenesh software (Softberry), functional annotation was carried out with Blast2GO software (BioBam) and tRNAs and rRNAs were predicted usingtRNAscan-SE and HMM-rRNA tools from WebMGA server. The paired-end libraries produced 31117554 reads with a total of 3142872954 bp, representing an average coverage of 77.39 X. The genome was assembled into 419 scaffolds with a total length of 35.18 Mbp (1000 bp; N50=498048 bp) and an overall G+C content of 50.5 %. A total of 8998 protein-coding genes were predicted, whose functional annotation is discussed. Additionally, 94 tRNAs and 44 rRNAs were found. This draft genome sequence, the first available forS. lycopersici, represents a new resource for further research into the taxonomy, biology and phytopathology of this plant pathogen.
format Objeto de conferencia
Objeto de conferencia
author Franco, Mario Emilio Ernesto
López, Silvina Marianela Yanil
Medina, Rocío
Saparrat, Mario Carlos Nazareno
Balatti, Pedro Alberto
author_facet Franco, Mario Emilio Ernesto
López, Silvina Marianela Yanil
Medina, Rocío
Saparrat, Mario Carlos Nazareno
Balatti, Pedro Alberto
author_sort Franco, Mario Emilio Ernesto
title Draft Genome Sequence of the Plant-Pathogenic Fungus <i>Stemphylium lycopersici</i> Strain CIDEFI-216
title_short Draft Genome Sequence of the Plant-Pathogenic Fungus <i>Stemphylium lycopersici</i> Strain CIDEFI-216
title_full Draft Genome Sequence of the Plant-Pathogenic Fungus <i>Stemphylium lycopersici</i> Strain CIDEFI-216
title_fullStr Draft Genome Sequence of the Plant-Pathogenic Fungus <i>Stemphylium lycopersici</i> Strain CIDEFI-216
title_full_unstemmed Draft Genome Sequence of the Plant-Pathogenic Fungus <i>Stemphylium lycopersici</i> Strain CIDEFI-216
title_sort draft genome sequence of the plant-pathogenic fungus <i>stemphylium lycopersici</i> strain cidefi-216
publishDate 2015
url http://sedici.unlp.edu.ar/handle/10915/66623
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